Expression of intercellular adhesion molecule-1 in U937 foam cells and inhibitory effect of imperatorin  

U937泡沫细胞中细胞间粘附分子-1的表达及欧芹素乙的抑制作用(英文)

作　　者：杨鹏远[1] 芮耀诚 李凯 黄兴华[3] 蒋建明[3] 余龙[3] 

机构地区：[1]第二军医大学药学院药理教研室 [2]长海医院胸心外科,上海中国200433 [3]复旦大学遗传学研究所,上海中国200433

出　　处：《Acta Pharmacologica Sinica》2002年第4期327-330,共4页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of CHina, № 39670832, and also supported by the shanghai-Unilever Research & Development Fund.

摘　　要：AIM: To investigate the expression level of intercellular adhesion molecule-1 (ICAM-1) in a macrophage-derived foam cell model from human U937 cell line and the inhibitory effect of imperatorin (IMP) on the ICAM-1. METHODS: U937 cells were incubated with oxidized low density lipoprotein (ox-LDL) 80 mg/L for 48 h and a macrophage-derived foam cell model was established. The medium was pretreated with different concentrations of IMP (0, 25, 50, 100 μmol/L). ICAM-1 protein expression in cells was measured with Western blotting; ICAM-1 mRNA level in cells was measured by Northern blotting. RESULTS: After incubated with ox-LDL, ICAM-1 expression level increased greatly. The increase in ICAM-1 protein level and mRNA level was estimated to be about 15-fold and 10-fold. When the cells were pretreated with imperatorin ( 50, 100 μmol/L), the increase of ICAM-1 in foam cells were remarkably inhibited. Especially when pretreated with IMP 100μmol/L, the ICAM-1 protein level decreased by 79 % and the mRNA level decreased by 74 % each compared to the level of foam cells. CONCLUSION: After incubated with ox-LDL in vitro, the U937 foam cells showed an enhanced ICAM-1 expression compared with normal U937 cells. IMP could inhibit the expression of ICAM-1 in U937 foam cells.目的:研究在人类单核细胞系U937泡沫细胞中,细胞间粘附分子-1(ICAM-1)的表达水平,观测欧芹素乙(欧前胡内酯,imperatorin,IMP)对ICAM-1表达的抑制作用.方法:将U937细胞与80mg/L氧化低密度脂蛋白孵育48h,建立U937泡沫细胞模型.在培养基中预加入不同浓度的IMP(0,25,50,100μmol/L).采用Western blotting检测ICAM-1的蛋白表达;采用Northern blotting检测ICAM-1的mRNA水平.结果:泡沫细胞中ICAM-1的表达显著高于正常U937细胞.ICAM-1的蛋白和mRNA水平分别是正常U937的15和10倍.经IMP 50和100μmol/L预处理后,泡沫细胞中ICAM-1的高表达被显著抑制.当IMP浓度达到100μmol/L时,ICAM-1的蛋白水平降低了79％,mRNA水平降低了74％.结论:经氧化低密度脂蛋白孵育后,U937泡沫细胞中ICAM-1呈现高表达,IMP能显著抑制这种表达.

关 键 词：foam cells intercellular adhesion molecule-1 IMPERATORIN LDL lipoprotein Western blotting Northern blotting 

分 类 号：R96[医药卫生—药理学]