Reduction in monocyte chemoattractant protein-1 mRNA expression in peripheral blood mononuclear cells of diamorphine addicts  

乙酰吗啡成瘾者外周血单个核细胞中单核细胞趋化蛋白-1 mRNA表达的抑制(英文)

作　　者：宋烈昌 宋畅 曾钧 熊茂林 娄宁 

机构地区：[1]第一军医大学卫生学系分子药理实验室

出　　处：《Acta Pharmacologica Sinica》2002年第4期336-338,共3页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China, № 39670828.

摘　　要：AIM: To investigate whether there is a significant difference in the monocyte chemoattractant protein-1 (MCP-1 ) mRNA expression between diamorphine (heroin) addicts and normal volunteers. METHODS: Expression of MCP-1 mRNA in the peripheral blood mononuclear cells of diamorphine addicts and normal volunteers was examined by reverse transcription-polymerase chain reaction (RT-PCR) with β-actin as an internal standard. Sequencing of RT-PCR products was performed to confirm the specificity of these products in MCP-1 gene composition. RESULTS: The relative MCP-1 mRNA expression ratios (MCP-1/β-actin) in the peripheral blocxl mononuclear cells of normal volunteers control group and diamorphine addicts group were 0.47±0.12 (n = 15) and 0.21 ±0.09 (n = 21), respectively, and there was a significant difference ( P < 0. 05). CONCLUSION: The significant reduction of MCP-1 mRNA expression in the peripheral blood mononuclear cells of diamorphine addicts may be one of the mechanisms for the high incidence of severe infectious diseases, including AIDS, among diamorphine addicts.目的:探究乙酰吗啡(海洛因)成瘾者单核细胞趋化蛋白-1(MCP-1)基因表达状况是否与正常人有区别.方法:以β-actin为内标准物,用逆转录聚合酶链式反应(RT-PCR)分别检测乙酰吗啡成瘾者和正常志愿者外周血单个核细胞中MCP-1 mRNA表达状况,并对RT-PCR产物进行测序,以证实其特异性.结果:外周血单个核细胞中MCP-1 mRNA表达的相对水平,正常志愿者对照组为0.47±0.12(n=15);乙酰吗啡成瘾组为0.21±0.09(n=21),两组间差异显著(P<0.05).结论:乙酰吗啡成瘾者外周血单个核细胞中MCP-1 mRNA的表达显著被抑制;这可能是乙酰吗啡成瘾者群体中包括AIDS等严重感染性疾病发病率显著增高的机制之一.

关 键 词：DIAMORPHINE monocyte chemoattractant protein-1 mononuclear cells habitation (psychophy- siology) 

分 类 号：R749.61[医药卫生—神经病学与精神病学]