miR-218在大肠癌中的表达及其临床意义  

Expression and Clinical Significance of miR-218 in Colorectal Cancer

作　　者：许文剑[1] 刘明[2] 苏秀兰[3] 

机构地区：[1]内蒙古医科大学附属人民医院普外科,呼和浩特010020 [2]内蒙古医科大学附属医院肿瘤外科,呼和浩特010050 [3]内蒙古医科大学附属医院临床医学研究中心,呼和浩特010050

出　　处：《医学综述》2015年第9期1674-1676,共3页Medical Recapitulate

基　　金：国家自然科学基金(81160254);内蒙古医科大学附属医院重大项目(NYFYZD2010008)

摘　　要：目的研究miR-218在大肠癌与正常组织中表达的差异。方法应用实时荧光定量聚合酶链反应检测60例大肠癌及相应正常组织中miR-218的表达水平,计算其相对表达量。结果60例大肠癌组织中miR-218的相对表达量为0.33±0.12,显著低于对照组,miR-218的表达差异有统计学意义(P<0.05);miR-218在不同性别、年龄、肿瘤大体形态、组织学类型、临床分期、浸润深度、肿瘤分化程度上表达差异无统计学意义(P>0.05),有淋巴结转移较无淋巴结转移者表达量低(P<0.05)。结论 miR-218在大肠癌中呈低表达,其表达水平越低可能提示淋巴结转移越早,miR-218可能作为抑癌基因参与肿瘤的发生、发展。Objective To study the expression levels of miR-218 in colorectal cancer and normal tis- sues. Methods Using real-time quantitative polymerase chain reaction to detect the expression levels of miR-218 in 60 cases of colorectal cancer and the corresponding normal tissues, and calculate its relative ex- pression. Results The relative expression of miR-218 in the tissues of 60 patients with colorectal cancer was 0.33 ＋ 0. 12, it was significantly lower than the control group, the difference was statistically significant （P 〈0. 05） ;there was no statistically significant difference in the expression of miR-218 in the general form of gender, age, tumor histological type, clinical stage, depth of invasion, and the degree of tumor differentiation （ P 〉 0. 05 ） ; there was lower expression in lymph node metastasis than in those without lymph node metasta- sis （P 〈 0. 05 ）. Conclusion miR-218 shows low expression in colorectal cancer, Its lower expression levels may indicate earlier lymph node metastasis, miR-218 may act as a tumor suppressor gene involved in tumor development.

关 键 词：大肠癌 MIR-218 实时荧光定量聚合酶链反应 

分 类 号：R735.35[医药卫生—肿瘤]