SNCG在肝癌高/低淋巴道转移细胞株中的差异性表达  

SNCG expression in high and low lymph tract metastasis potential hepatocellular carcinoma cells

作　　者：魏元怡[1] 张军[1] 王波[1] 黄玉红[1] 王梅[1] 王璐[1] 唐建武[1] 

机构地区：[1]大连医科大学病理学与法医学教研室,辽宁大连116043

出　　处：《现代肿瘤医学》2015年第9期1177-1179,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(No.3077246 8;No.81071725);辽宁省教育厅资助项目(No.2008225010-3;No.2007T024)

摘　　要：目的:研究SNCG蛋白在小鼠肝癌高/低淋巴道转移潜能细胞株中的定位和表达差异。方法:应用免疫细胞化学、Western blot方法检测SNCG蛋白在小鼠肝癌高/低淋巴道转移潜能细胞株Hca-F/Hca-P的定位情况和表达差异。结果:SNCG蛋白在Hca-F和Hca-P细胞株中均主要定位于细胞浆,细胞核区域也可见少量蛋白表达。SNCG在Hca-F中的表达[染色强度为(+++)]强于在Hca-P中的表达[染色强度为(++)]。检测到一条SNCG特异性蛋白条带,在Hca-F细胞中表达量为Hca-P细胞中的1.9倍。结论:SNCG的高表达可能与高淋巴道转移潜能有关。Objective：To investigate the location and expression of SNCG in mouse hepatocarcinoma cells Hca -F,Hca - P. Methods：We tested the Hca - F and Hca - P cells with HRP - immunocytochemistry and Western blot for location and expression of SNCG. Results：SNCG protein locates in cytoplasma mainly in Hca - F and Hca - P cells. It also locates in nuclear. The strength of expression was higher in Hca - F than Hca - P. The intensity of Hca -F was（ ＋ ＋ ＋ ）,and that of Hca - P（ ＋ ＋ ）. We found a specific band. The expression in Hca - F was 1. 9 times of that in Hca - P and the data had significantly difference（P = 0. 016）. Conclusion：High expression of SNCG is pres-ent in high metastasis potential hepatocarcinoma cell. SNCG may be used as a predictive biomarker for lymph tract metastasis.

关 键 词：SNCG 肝癌 淋巴道转移 

分 类 号：R730[医药卫生—肿瘤] R735.7[医药卫生—临床医学]