大鼠腹部手术应激后血清miRNA表达谱的差异分析  

Altered expression levels of miRNAs in rat serum post abdominal operation

作　　者：千年松[1,2] 杨凡[3] 印凡[1,4] 赵宏[1] 

机构地区：[1]解放军总医院海南分院肿瘤内科,海南三亚572000 [2]解放军总医院肿瘤中心肿瘤内二科,北京100853 [3]第四军医大学西京医院手术室,陕西西安710032 [4]解放军总医院肿瘤中心实验室,北京100853

出　　处：《现代肿瘤医学》2015年第9期1201-1204,共4页Journal of Modern Oncology

基　　金：北京市科技新星资助计划(编号:xx2013107);三亚市医疗卫生科技创新项目(编号:YW1304);解放军总医院临床科研扶持基金(编号:2012FC-CXYY-4008)

摘　　要：目的:应用miRNA芯片技术建立大鼠腹部手术应激后血清miRNA差异表达谱,从中发现与早期手术创伤后应激相关的miRNAs。方法:建立大鼠部分肝切除手术模型。检测手术前后大鼠血清中ALT、AST和CRP浓度水平及肝脏病理改变,评估腹部创伤后大鼠应激情况。采用miRNA表达谱芯片筛选手术前后大鼠血清中差异表达的miRNAs,并采用实时荧光定量RT-PCR(real-time RT-PCR)进行验证。采用生物信息学软件(MiRand和Target Scan)预测候选miRNA的靶基因。结果:血清miRNA表达谱出现明显改变,24个miRNAs在2/3部分肝切除术(PH)后24h大鼠血清中表达明显升高,特别是miR-9,其在2/3PH术后24h大鼠血清中的表达水平为术前的50多倍,real-time RT-PCR检测miR-9的结果与芯片结果相符。通过生物信息学预测,CDH1、E-cadherin、MTHFD2、PDYN、MCPIP1、BCL2L11、CMA1、Map3k1等可能是miR-9的靶基因。结论:腹部手术创伤后,大鼠血清表达谱发生明显变化,提示miRNA可能参与调控创伤后应激反应。Objective：To screen and identify the differential expression of miRNAs in rat serum post traumatic in-jury by the miRNA microarray technique. Methods：We chose partial hepatectomy（PH）as traumatic injury model. The serum ALT,AST,CRP and histological changes of the liver were measured to evaluate the severity of trauma. The miRNA microarray was used to assess the level and composition of serum miRNAs. The target genes of candidate miR-NA were predicted by bioinformatics software（MiRand and TargetScan）. Results：There was significantly differential expression of miRNAs in rat serum post traumatic injury（24 miRNAs were more than 2 - fold up - regulated）. Espe-cially,the expression of miR - 9 showed the highest up - regulated（more than 50 - fold）. The results showed that CDH1,E - cadherin,MTHFD2,PDYN,MCPIP1,BCL2L11,CMA1,Map3k1 may be the target genes of miR - 9. Con-clusion：Injury caused by abdominal operation could make significant change in the spectra and levels of serum miR-NAs,suggesting that miRNAs may be involved in regulating the stress response post trauma.

关 键 词：MIRNA芯片 血清 腹部创伤 部分肝切除 

分 类 号：R73-3[医药卫生—肿瘤]