条件复制腺病毒携带白细胞介素24增强Hela细胞放射敏感性  

A conditionally replicating adenovirus carrying interleukin-24 increased the effect of radiation on Hela cells

作　　者：陈亚平[1] 姜晓晓[2] 冯守信[3] 蒋冠[4] 

机构地区：[1]徐州医学院附属医院肿瘤科,江苏徐州221000 [2]徐州医学院附属医院泌尿外科,江苏徐州221000 [3]徐州医学院附属医院立体定向放疗科,江苏徐州221000 [4]徐州医学院江苏省肿瘤生物治疗重点实验室,江苏徐州221000

出　　处：《现代肿瘤医学》2015年第9期1205-1209,共5页Journal of Modern Oncology

摘　　要：目的:观察条件复制性腺病毒(ZD55)携带白介素24(ZD55-IL-24)单独与联合放射治疗对宫颈癌Hela细胞的治疗效果。方法:体外培养宫颈癌Hela细胞,分成PBS组、放射治疗组、ZD55-IL-24组、ZD55-IL-24联合放射治疗组。采用MTT法检测细胞生长抑制率,Annexin V-FITC/PI双染法检测细胞凋亡率,流式细胞仪检测细胞活性氧ROS水平,Western blot检测凋亡蛋白Bax、Bcl-2表达。结果:MTT法显示PBS组、放射治疗组、ZD55-IL-24组、ZD55-IL-24联合放射治疗组抑制率分别为(7.28±1.99)%、(45.16±4.12)%、(33.23±2.63)%、(85.27±2.03)%,ZD55-IL-24联合放射治疗组细胞抑制率大于其他三组(P<0.05)。Annexin V-FITC/PI双染法显示ZD55-IL-24联合放射治疗组细胞凋亡率为(72.82±4.57)%,与PBS组(7.40±0.74)%,放射治疗组(33.60±3.27)%,ZD55-IL-24组(24.66±5.36)%相比,差异有统计学意义(P<0.05)。流式细胞仪检测PBS组、放射治疗组、ZD55-IL-24组、ZD55-IL-24联合放射治疗组细胞ROS率分别为(8.37±1.27)%、(27.97±2.22)%、(21.60±1.12)%、(43.57±2.61)%,ZD55-IL-24联合放射治疗组与其他三组相比,差异有统计学意义(P<0.05)。Western blot显示ZD55-IL-24联合放射治疗组Bax蛋白表达明显增加,Bcl-2蛋白明显减少,差异有统计学意义(P<0.05)。结论:条件复制性腺病毒携带白介素24可增强宫颈癌Hela细胞放射敏感性。Objective：To evaluate the effects of the conditionally replicating adenovirus carrying interleukin - 24 combined with radiation on cervical cancer Hela cell. Methods：Hela cells were divided into four groups：PBS group, radiation group,ZD55 - IL - 24 group and ZD55 - IL - 24 with radiation group. The cell viability was assessed by MTT assay. The apoptotic rate of cells was assessed by Annexin V - FITC/ PI assay. The expression of Bax,Bcl - 2 was detected by Western blot. Results：PBS group,cell viability of radiation group,ZD55 - IL - 24 group and ZD55 -IL - 24 with radiation group were（7. 28 ± 1. 99）% ,（45. 16 ± 4. 12）% ,（33. 23 ± 2. 63 ）% and（85. 27 ± 2. 03）% , which reveals cell viability of ZD55 - IL - 24 with radiation group was higher than other groups（P 〈 0. 05）. Annexin V - FITC / PI revealed that the apoptotic rate of ZD55 - IL - 24 with radiation was higher than other groups（P〈 0. 05）. ROS level of ZD55 - IL - 24 with radiation was higher than other groups（P 〈 0. 05）. The expression of Bax in ZD55 - IL - 24 with radiation group was higher than in other groups（P 〈 0. 05）,while the expression of Bcl - 2 in ZD55 - IL - 24 with radiation group was lower than in other groups（P 〈 0. 05）. Conclusion：The conditionally repli-cating adenovirus carrying interleukin - 24 can increase radiosensitivity of cervical cancer Hela cell.

关 键 词：白介素24 放射治疗 宫颈癌 

分 类 号：R730.55[医药卫生—肿瘤] R737.33[医药卫生—临床医学]