下调RPL23对胃癌耐药细胞SGC7901/ADR药物敏感性的影响  

The roles of RPL23 knock-down on drug resistance in gastric cancer cell line SGC7901/ADR

作　　者：于海燕[1] 张志勇[1] 王凯[1] 刘刚[1] 张德新[1] 

机构地区：[1]第四军医大学西京消化病医院肿瘤生物学国家重点实验室,陕西西安710032

出　　处：《现代肿瘤医学》2015年第10期1340-1343,共4页Journal of Modern Oncology

基　　金：国家自然科学基金面上项目(编号:81172069)

摘　　要：目的:检测人胃癌细胞系SGC7901及其阿霉素(ADR)耐药细胞系SGC7901/ADR中RPL23的表达差异,探讨胃癌细胞中RPL23表达对ADR耐药的影响。方法:q PCR检测RPL23在SGC7901/ADR与SGC7901两种细胞系中的表达差异;MTT法检测下调RPL23后SGC7901/ADR细胞对ADR的敏感性;流式细胞仪检测下调RPL23后对细胞凋亡的影响;Western Blot检测凋亡相关分子Bcl-2和Caspase-3蛋白的表达。结果:q PCR结果显示,RPL23在SGC7901/ADR细胞系中的表达高于其在SGC7901细胞系中的表达(P<0.001)。MTT法结果显示,下调RPL23后,在SGC7901/ADR细胞系中ADR的IC50明显升高(P<0.001)。凋亡检测结果显示,下调RPL23后,SGC7901/ADR细胞凋亡率明显增加(P<0.001)。Western Blot结果显示,下调RPL23后,细胞中Caspase-3表达明显升高,Bcl-2表达明显降低。结论:RPL23在SGC7901/ADR中的表达高于SGC7901细胞,下调RPL23表达可提高其对ADR的敏感性并促进细胞凋亡,提示RPL23可能通过作用于Bcl-2调节细胞凋亡。Objective：To evaluate the influence of RPL23 on the drug resistance and cell apoptosis in human gastric cancer cell line SGC7901/ADR. Methods： The mRNA expression levels of RPL23 in SGC7901 and SGC7901/ ADR were determined by Real - time PCR（ RT - PCR） assay. The influence of RPL23 silencing on the IC50 and cell apoptosis of gastric cancer cell line SGC7901/ADR were detected using MTT and FACS assay. Results：RPL23 mRNA levels were up -regulated in gastric cancer cell SGC7901/ADR compared with SGC7901 cell（ P 〈0.001 ） ;MTT assay and FACS assay showed that the IC50 of ADR and apoptosis rate were decreased after down - regulation of RPL23 in the gastric cancer cell line SGC7901/ADR （P 〈 0. 001 ）. Conclusion： RPL23 silencing could reverse the phenotype of multidrug resistance in gastric cancer cell line SGC7901 ADR.

关 键 词：胃癌 RPL23 耐药 

分 类 号：R735.2[医药卫生—肿瘤]