三氧化二砷增强TRAIL对肺癌移植瘤抑制作用的研究  

Inhibitory action and mechanism of TRAIL combined with ATO on tansplanted human lung cancer in nude mice

作　　者：赵雪强[1] 蒋碧佳[1] 银建华[1] 唐碧芸 黄秀兰[1] 李文翠[1] 

机构地区：[1]桂林医学院附属医院临桂分院呼吸内科,广西桂林541199

出　　处：《现代肿瘤医学》2015年第10期1352-1355,共4页Journal of Modern Oncology

基　　金：广西卫生厅立项课题项目(编号:Z2013490)

摘　　要：目的:探讨肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor related apoptosis inducing ligand,TRAIL)联合三氧化二砷(arsenic trioxide,ATO)对裸鼠肺癌移植瘤的抗肿瘤作用及可能机制。方法:将裸鼠肺癌移植瘤模型随机分为生理盐水组、TRAIL组、ATO组及TRAIL+ATO组,称瘤重并计算抑瘤率,用RTPCR检测NF-κB、Caspase-3、Survivin基因表达变化。结果:与生理盐水组比较,ATO组对裸鼠肺癌移植瘤具有抑制作用,联合用药具有协同增效作用,抑制NF-κB表达,下调Survivin mRNA,上调Caspase-3的表达。结论:ATO可能通过NF-κB通路,下调Survivin,上调Caspase-3增强TRAIL对裸鼠肺癌移植瘤的抗肿瘤作用。Objective：To study the inhibitory action and mechanism of TRAIL combined with ATO on tansplanted human lung cancer in nude mice. Methods： Nude mice bearing A549 lung cancer were randomly divided into normal saline group, TRAIL group, ATO group and TRAIL ＋ ATO group. Tumor tissues were weighted and inhibitory rate was observed. Nuclear factor kappa B （ NF - κB）, Caspase - 3, Survivin mRNA were assayed by reverse transcriptase - polymerase chain reaction（ RT -PCR）. Results：Compared with NS, both ATO and ATO ＋ TRAIL obviously inhibited the growth of tansplanted human lung cancer in nude mice. They had a synergistic effect （P 〈 0.05）, down - regulated the expression of NF - KB and Survivin, up - regulated the expression of Caspase - 3 （ P 〈 0.05 ）. Conclusion ： ATO combining with TRAIL can synergistic inhibit the gowth of tansplanted human lung cancer in nude mice, possible mechanism may be inhibition of NF -κB signaling pathway and down - regulation of Survivin expression, up - regulation of Caspase - 3 expression.

关 键 词：三氧化二砷 肿瘤坏死因子相关凋亡诱导配体 肺肿瘤 NF-ΚB 

分 类 号：R734.2[医药卫生—肿瘤]