太白银莲花皂甙-1抑制人脑胶质瘤U251细胞生长的实验研究  

Anemone taipaiensis saponins-1 inhibits proliferation of human glioma U251 cells: an experimental study

作　　者：李娟[1,2] 程光[1] 李三中[1] 费舟[1] 章翔[1] 汤海峰[3] 张赟[4] 汤池[2] 甄海宁[1] 陈涛[1] 罗鹏[1] 殷安安[1] 

机构地区：[1]第四军医大学西京医院全军神经外科研究所,陕西西安710032 [2]第四军医大学生物医学工程系,陕西西安710032 [3]第四军医大学西京医院药剂科,陕西西安710032 [4]第四军医大学基础部免疫教研室,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2015年第2期109-113,共5页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(30873402);陕西省自然基金资助项目(2012JM4010)

摘　　要：目的研究太白银莲花皂甙-1对人脑胶质瘤U251细胞生长的影响及其机制。方法采用四甲基偶氮唑蓝(MTT)比色法检测太白银莲花皂甙-1对U251细胞增殖的影响;应用倒置显微镜和Hochest33342荧光染色、透射电子显微镜观察细胞形态的变化;通过逆转录酶多聚酶链反应(RTPCR)检测与凋亡相关基因Bcl-2 mRNA和Bax mRNA水平的表达变化,Western blot分析Bcl-2和Bax在胶质瘤U251细胞中蛋白的表达情况。结果 U251细胞经太白银莲花皂甙-1诱导后,细胞的增殖明显受到抑制,通过RT-PCR结果证实Bcl-2 mRNA在太白银莲花皂甙-1作用下明显呈低表达而Bax mRNA的表达随时间延长逐渐增高,Western blot检测结果显示太白银莲花皂甙-1作用后,下调抑凋亡蛋白Bcl-2的表达并激活了促凋亡蛋白Bax的表达。结论太白银莲花皂甙-1明显抑制U251细胞的生长活性,且能引起胶质瘤细胞大量凋亡,具有显著的抗肿瘤作用。Objective The effect of anemone taipaiensis saponins-1 on proliferation of human U251 glioma cells and its mechanism are investigated. Methods Methyl thiazolyl tetrazolium （MTT） assay was used to detect the effect of anemone taipaiensis saponins-1 on the proliferation of U251 glioma cells; the cell morphological changes were observed by Hechest33342 staining under inverted microscope and transmission electron microscopy; and the expression of mRNA and protein levels of Bcl-2 and Bax in U251 glioma cells were examined by reverse transcriptase-polymerase chain reaction （RT-PCR） and Western blot. Results Anemone taipaiensis saponins-1 significantly inhibited the cell viability of U251 glioma cells, and the results of RT-PCR and Western blot showed that anemone taipaiensis saponins-1 down-regulated the expression of anti-apoptotic protein Bcl-2 and activated the pro-apoptotic protein Bax. Conclusion Anemone taipaiensis saponins-1 with its anti-tumor activities can effectively inhibit the cell viability of U251 glioma cells and induce the cell apoptosis.

关 键 词：太白银莲花皂甙-1 胶质瘤U251 细胞凋亡 

分 类 号：R739.41[医药卫生—肿瘤]