抑制容量性钙内流对U87侵袭及迁移力的影响  

Effects of capacitive calcium entry inhibition on migration and invasion of U87 cells

作　　者：石子璇 李军昌[1] 谢娟[1] 王宗仁[1] 

机构地区：[1]第四军医大学西京医院中医科,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2015年第2期114-117,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(81273879)

摘　　要：目的探讨抑制容量性钙内流(CCE)对胶质瘤U87细胞侵袭及迁移力的影响。方法采用免疫印迹(Western blot)检测基质相互作用分子1(STIM1)RNA干涉(RNAi)后U87细胞STIM1表达变化;活细胞钙成像检测CCE抑制剂(SKF-96365)处理及STIM1 RNAi后U87细胞CCE的改变;细胞划痕和Transwell侵袭实验检测CCE对U87细胞侵袭及迁移力的影响。结果 SKF-96365处理及STIM1RNAi均能明显抑制U87细胞的CCE(P<0.05);与对照组比较,抑制CCE后U87细胞迁移及侵袭能力明显降低(P<0.05)。结论抑制CCE可有效抑制U87细胞迁移及侵袭力,其机制可能与调控细胞内钙稳态相关。Objective The effects of capacitive calcium entry （CCE） inhibition on migration and invasion of glioma cell line U87 cells are discussed. Methods Stromal interaction molecule 1 （STIM1） expression of U87 cells treated with STIM1 RNA interfere （RNAi） were detected by Western blot. The cell cytosolic Ca^2＋ concentration was measured by calcium imaging after SKF-96365 or STIM1 RNAi treatment. The migration and invasion ability were examined by in vitro wound healing assay and Transwell invasion assay, respectively. Results Calcium imaging showed SKF-96365 or STIM1 RNAi insults inhibited the CCE of U87 cells （P 〈0.05）. Compared with control group, the migration and invasion of U87 cells treated with SKF-96365 or STIM1 RNAi were decreased obviously （P 〈0.05）. Conclusion CCE inhibition suppressed the migration and invasion of U87 cells. The suppression might be associated with regulation of calcium homeostasis.

关 键 词：容量性钙内流 胶质瘤 基质相互作用分子1 迁移 侵袭 

分 类 号：R730[医药卫生—肿瘤]