骨髓间充质干细胞对血管性痴呆大鼠的保护作用  

Protection of bone marrow mesenchymal stem cell on the rat model of vascular dementia

作　　者：樊才瑞 刘卫平[1] 龙乾发[2] 汪凯[3] 黑悦 罗强[1] 

机构地区：[1]第四军医大学西京医院神经外科,陕西西安710032 [2]西安市中心医院神经外科,陕西西安710003 [3]解放军第401医院神经外科,山东青岛266071

出　　处：《中华神经外科疾病研究杂志》2015年第2期146-149,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：陕西省科技统筹基金资助项目(2013KTCL03-08);社会发展引导计划-医学研究基金资助项目(SF1423-2)

摘　　要：目的探讨骨髓间充质干细胞(BMSC)对血管性痴呆大鼠的保护作用。方法雄性SD大鼠随机分为正常组、模型组和移植组。采用双侧颈总动脉永久性结扎建立大鼠血管性痴呆(Va D)模型,模型建立24 h后,尾静脉移植第三代BMSC 3×106个。于移植结束后第14 d取海马组织Western blot分析缺氧诱导因子(HIF-1α),血管内皮生长因子(VEGF)和Caspase-3表达量的变化,免疫荧光分析血管密度的变化。结果移植组干预效果显著,VEGF表达量较模型组明显升高(P<0.05),Caspase-3表达量较模型组显著下降(P<0.05),且血管密度较模型组明显增高(P<0.05),而HIF-1α表达量则无明显差异(P>0.05)。结论骨髓间充质干细胞促进了血管生成,对血管性痴呆大鼠有一定的保护作用。Objective The neuroprotection of bone marrow mesenchymal stem cell （BMSC） on the bilateral common carotid artery occlusion model （BCCAO） is discussed.Methods Male Sprague-Dawley rats were randomly divided into three groups： normal group, vascular dementia （VaD） group and VaD-BMSC group. Rats in the VaDBMSC group were administered 3 × 10^6 BMSC via tail vein infusion at 24 h after BCCAO. The rats were sacrificed at 14 d after transplantation. Western blot analysis was used to analyze the expression of hypoxia-inducible factor-lot （HIF-1α）, vascular endothelial growth factor （VEGF） and Caspase-3. Immunofluorescence analysis was used to analyze the bloed vessel density.Results Compared with the VaD group, the expression of VEGF in the VaD-BMSC group was significantly elevated （P 〈0.05）, the expression of Caspase-3 in the VaD-BMSC group was significantly decreased （P 〈0. 05）, the blood vessel density of VaD-BMSC group was significantly higher than that of VaD group （P 〈0. （15） and there was no significant difference of HIF-1α between the VaD group and VaD-BMSC group （P〉0.05）.Conclusion BMSC could promote the angiogenesis and play a protective role on the rat model of vascular dementia.

关 键 词：骨髓间充质干细胞 血管性痴呆 细胞移植 血管内皮生长因子 血管生成 

分 类 号：R743.9[医药卫生—神经病学与精神病学]