视神经减压及地塞米松干预视神经损伤模型兔视网膜Nogo-A Caspase-3的变化  

Changes of Nogo-A and Caspase-3 in Rabbit Retina of Optic Nerve Injury Model by Optic Nerve Decompression and Dexamethasone Intervention

作　　者：林琳[1] 王茜[2] 买买江·阿不力孜[1] 张永辉[1] 李大志[1] 依马木·依达依吐拉[1] 昝向阳[1] 栾新平 

机构地区：[1]新疆医科大学附属中医院神经外科,乌鲁木齐830000 [2]新疆医科大学公共卫生学院,乌鲁木齐830011 [3]新辐医科大学第二附属医院神经外科,乌鲁木齐830063

出　　处：《中国医药导刊》2015年第4期390-396,共7页Chinese Journal of Medicinal Guide

基　　金：新疆维吾尔自治区自然科学基金项目(200821137);课题名称:视神经损伤后的病理溃变及手术的药物干预的实验研究

摘　　要：目的:观察兔视神经损伤模型在不同影响因素下的视网膜Nogo-A及Caspase-3受体表达。方法:成年健康白兔96只,其中6只为空白对照组,18只为假伤组,72只进行视神经损伤模型制作,随机分为单纯视神经损伤组,地塞米松组、视神经减压组、视神经减压联合地塞米松组,每组分为1天、7天、21天亚组,每亚组6只。观察视y网膜神经节细胞Nogo-A,Caspase-3受体表达,进行统计学分析。结果:急性视神经损伤可以促使Nogo-A,Caspase-3阳性表达上调,三种治疗与单纯损伤组在1天时间点有差异(P<0.05),且具统计学意义。与单纯损伤组在7天、21天时间点均无差异(P>0.05),且无统计学意义。结论:Nogo-A,Caspase-3可能在视神经损伤的神经再生过程中发挥着重要作用。三种治疗无明确疗效。Objective： To observe the pathological expression of Nogo-A,Caspase-3 receptor in retinal of rabbit optic nerve injury model under different impact factor.Methods：96 healthy adult rabbits,among these six is for blank control group,6 for the sham group,to exclude influence of surgical factors on optic nerve and retinal,on the remaining 72 rabbits were randomly divided into four large groups： model group,simple optic nerve injury group,dexamethasone effects of medication group,optic nerve decompression effect group,the optic nerve decompression combined with dexamethasone therapy combined effect of group,and each group was divided into three subgroups,r espeetively,after 1 day group,7 days group and 21 days groups,6 for each sub-group.Outcome measures：observation of optic nerve,retinal Nogo-A,Caspase-3 receptor immunohistoehemistry pathology results of immunohistochemistty, correlation analysis of the effects of optic nerve decompression and nerve model for dexamethasone for the model.and Statistical analysis.Result：Acute optic nerve damage can promote Nogo-A,Caspase-3 upregulation.Optic nerve decompression and decompression dexamethasone dexamethasone three treatment groups with simple injury time points in one day there are differences （P〈0.05）,and possessed statistically significant.With simple injury in 7 days,21 days time points were no differences （P〉0.05）,and no statistical significance.Conelusion：Acute optic nerve damage can promote Nogo.A upregulation,indicating that Nogo-A,Caspase-3 plays an important role in optic nerve regeneration after optic nerve injury.Current clinical treatments has no definite effectiveness.

关 键 词：视神经损伤 视网膜神经节细胞 Nogo-A受体 Caspase-3受体 

分 类 号：R774.6[医药卫生—眼科]