Neuroprotective effect of interleukin-6 regulation of voltage-gated Na^+ channels of cortical neurons is time-and dose-dependent  

作　　者：Wei Xia Guo-yi Peng Jiang-tao Sheng Fang-fang Zhu Jing-fang Guo Wei-qiang Chen 

机构地区：[1]Department of Interventional Radiology,First Affiliated Hospital,Shantou University Medical College [2]Department of Neurosurgery,First Affiliated Hospital,Shantou University Medical College [3]Department of Pathogenic Microbiology and Immunology,Shantou University Medical College

出　　处：《Neural Regeneration Research》2015年第4期610-617,共8页中国神经再生研究（英文版）

基　　金：supported by a grant from the National Natural Science Foundation of China,No.30972766,31170852,81001322,81172795,81173048;the Specialized Research Fund for the Doctoral Program of Colleges and Universities,No.20094402110004

摘　　要：Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorly understood. This study investigated the effects of 24 hour expo-sure of interleukin-6 on cortical neurons at various concentrations （0.1, 1, 5 and 10 ng/mL） and the effects of 10 ng/mL interleukin-6 exposure to cortical neurons for various durations （2, 4, 8, 24 and 48 hours） by studying voltage-gated Na＋ channels using a patch-clamp technique. Volt-age-clamp recording results demonstrated that interleukin-6 suppressed Na＋ currents through its receptor in a time- and dose-dependent manner, but did not alter voltage-dependent activation and inactivation. Current-clamp recording results were consistent with voltage-clamp recording results. Interleukin-6 reduced the action potential amplitude of cortical neurons, but did not change the action potential threshold. The regulation of voltage-gated Na＋channels in rat corti-cal neurons by interleukin-6 is time- and dose-dependent.Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorly understood. This study investigated the effects of 24 hour expo-sure of interleukin-6 on cortical neurons at various concentrations （0.1, 1, 5 and 10 ng/mL） and the effects of 10 ng/mL interleukin-6 exposure to cortical neurons for various durations （2, 4, 8, 24 and 48 hours） by studying voltage-gated Na＋ channels using a patch-clamp technique. Volt-age-clamp recording results demonstrated that interleukin-6 suppressed Na＋ currents through its receptor in a time- and dose-dependent manner, but did not alter voltage-dependent activation and inactivation. Current-clamp recording results were consistent with voltage-clamp recording results. Interleukin-6 reduced the action potential amplitude of cortical neurons, but did not change the action potential threshold. The regulation of voltage-gated Na＋channels in rat corti-cal neurons by interleukin-6 is time- and dose-dependent.

关 键 词：nerve regeneration brain injury inflammatory reaction INTERLEUKIN-6 voltage-gated Na＋ channel cortical neurons cerebrospinal fluid NEUROIMMUNOMODULATION neuroprotection action potential patch clamp neurophysiology NSFC grants neural regeneration 

分 类 号：R741[医药卫生—神经病学与精神病学]