CCXCKR、VEGF-A及VEGF-C在人直肠癌中的表达及其与转移的关系  

Expression of CCXCKR, VEGF-A and VEGF-C in rectal carcinoma and their relationship with lymph node metastasis

作　　者：罗建 于丽君[2] 孔露[3] 李东宁[3] 王昶[3] 李骢[3] 梁品[4] 

机构地区：[1]大连市甘井子区人民医院外科,辽宁省大连市116033 [2]大连医科大学组胚教研室,辽宁省大连市116044 [3]大连医科大学病理生理学教研室,辽宁省大连市116044 [4]大连医科大学附属第一医院胃肠外科,辽宁省大连市116011

出　　处：《世界华人消化杂志》2015年第11期1745-1754,共10页World Chinese Journal of Digestology

基　　金：大连市科技局社会发展项目;No.2010E15SF180;辽宁省教育厅科技计划项目;No.L2013340~~

摘　　要：目的:研究Chemo Centryx趋化因子受体(Chemo Centryx chemokine receptor,CCXCKR)、血管内皮生长因子(vascular endothelial growth factor,VEGF)A和C在直肠癌组织、癌旁组织、正常直肠组织中的表达,探讨其与淋巴结转移的关系.方法:应用免疫组织化学SP法检测50例手术切除的直肠癌组织、癌旁组织和11例正常直肠组织中CCXCKR、VEGF-A、VEGF-C的表达情况.将50例直肠癌患者根据有无淋巴结转移分为:淋巴结转移组(19例)与无淋巴结转移组,并采用免疫组织化学方法分别检测CCXCKR、VEGF-A及VEGF-C在有无淋巴结转移的直肠癌组织中的表达情况,以探讨CCXCKR、VEGF-A及VEGF-C与淋巴结转移的关系.结果:CCXCKR在正常直肠组织、癌旁组织中表达明显高于直肠癌组织(P<0.01),在淋巴结转移组CCXCKR的表达明显低于淋巴结未转移组(P<0.01).而直肠癌组织、癌旁组织中VEGF-A和VEGF-C表达阳性率明显高于正常直肠组织(P<0.01),其中在淋巴结转移组两者的表达明显高于淋巴结未转移组(P<0.05).VEGF-A和VEGF-C在直肠癌组织中的表达呈正相关(r=0.703,P<0.0l),而CCXCKR与V E G F-A及V E G F-C在直肠癌组织中的表达均分别呈负相关(r=-0.360,P<0.05、r=-0.326,P<0.05).结论:直肠癌中CCXCKR低表达、VEGF-A与VEGF-C高表达与直肠癌淋巴结转移密切相关,CCXCKR可能对直肠癌的发生和转移有抑制作用,CCXCKR、VEGF-A与V E G F-C联合检测,对直肠癌预后的判断具有实际意义.AIM： To detect the expressions of Chemo Centryx chemokine receptor（CCXCKR）, vascular endothelial growth factor（VEGF）-A and-C in rectal carcinoma, and to analyze their relationship with lymph node metastasis. METHODS： The immunohistochemical SP method was used to examine the expression of CCXCKR, VEGF-A and-C in 50 rectal carcinoma tissues, 50 para-cancerous tissues, and 11 normal rectal tissues. The 50 rectal carcinoma patients were divided into two groups： patients（n = 19） with lymph node metastasis and those without（n = 31）. The correlation between the expression of CCXCKR, VEGF-A and C and lymph node metastasis was analyzed.RESULTS： The expression of CCXCKR in both normal rectal tissues and para-cancerous tissues were significantly higher than that in rectal carcinoma tissues（P 〈 0.01）. The expression of CCXCKR in the rectal carcinoma group withly mphnode metastasis was significantly lower than that without lymphnode metastasis（P 〈 0.01）. Positive expression rates of VEGF-A and-C in rectal carcinoma tissues and para- canceroustissues were significantly higher than those in normal rectal tissues（P 〈 0.01）, and the expression of VEGF-A and-C in the rectal carcinoma group with lymph node metastasis was significantly higher than that without lymph node metastasis（P 〈 0.05）. There was a positive correlation between expression of VEGF-A and-C, and a significantly negative correlation between CCXCKR and VEGF-A or C（r =-0.360, P 〈 0.05; r =-0.326, P 〈 0.05）.CONCLUSION： Low expression of CCXCKR and high expression of VEGF-A and VEGF-C in rectal carcinoma are closely related to lymph nodemetastasis. CCXCKR probably can inhibit the occurrence and metastasis of rectal carcinoma. Combined detection of CCXCKR, VEGF-A and VEGF-C should be a useful prognostic indicator for rectal carcinoma patients.

关 键 词：直肠癌 Chemo Centryx趋化因子受体 血管内皮生长因子 转移 

分 类 号：R735.37[医药卫生—肿瘤]