宿主基质金属蛋白酶在乳牙牙本质胶原降解中的作用  

Effect of host matrix metalloproteinases on dentin collagen degradation of deciduous teeth

作　　者：徐珺[1] 宿颖[2] 杨东梅[3] 

机构地区：[1]首都医科大学口腔医学院综合科,北京100050 [2]首都医科大学口腔医学院口腔医学研究所,北京100050 [3]首都医科大学口腔医学院儿童口腔科,北京100050

出　　处：《北京口腔医学》2015年第2期89-91,共3页Beijing Journal of Stomatology

基　　金：北京市教育委员会科技发展计划面上项目(km200710025025)

摘　　要：目的研究乳牙牙本质中的基质金属蛋白酶在乳牙牙本质胶原降解中的作用。方法因滞留拔除的正常乳牙制备牙本质粉,分为空白组、醋酸氯己定组和6-去甲基-6-脱氧-4-去二甲氨基四环素(CMT-3)组;每组6份标本,每份标本50.0mg。各组标本置于脱矿液中6h,人工唾液中18h,进行p H循环,共7次。醋酸氯己定组和CMT-3组的脱矿液和人工唾液中分别加入0.2%醋酸氯己定和0.02%CMT-3。收集各组的脱矿液和人工唾液的上清液,用羟脯氨酸酶联免疫试剂盒分别检测各组脱矿液和人工唾液上清中的胶原降解量。结果空白组的脱矿液和人工唾液上清中的胶原降解量均高于醋酸氯己定组和CMT-3组,差异有统计学意义(P<0.05)。醋酸氯己定组和CMT-3组的胶原降解量差异无统计学意义(P>0.05)。结论乳牙牙本质中的基质金属蛋白酶活化后可降解牙本质胶原,使用基质金属蛋白酶抑制剂可抑制牙本质胶原的降解。Objective To investigate the effect of matrix metalloproteinases on dentin collagen degradation of deciduous teeth. Methods Retained deciduous teeth were extracted and crashed into powder which was divided into three groups, blank group, chlorhexidine group（MMPs inhibitor）and CMT-3 group（MMPs inhibitor）. There were 6 samples in each group, and each sample weighed 50. 0mg. All the samples were treated with demineralization solution for 6 hours and artificial saliva for 18 hours,and then pH cycle was conducted for a total of 7 times. The demineralization solution and artificial saliva of chlorhexidine group and CMT-3 group contained 0.2% chlorhexidine and 0. 02% CMT-3 separately. Supernatants of each sample was collected and the collagen degradation was detected by Hyp ELISA kit. Results The collagen degradation of blank group was more than chlorhexidine group and CMT-3 group in both demineralization solution and artificial saliva （ P 〈 0. 05 ）. No difference was found between chlorhexidine group and CMT-3 group （ P 〉 0. 05 ）. Conclusion MMPs in deciduous teeth dentin could lead to dentin collagen degradation after being actived. MMPs inhibitors may be useful in reducing the dentin collagen degradation.

关 键 词：基质金属蛋白酶 牙本质 胶原降解 

分 类 号：R781[医药卫生—口腔医学]