T-cadherin在肝细胞癌中的表达及其与肿瘤复发转移的关系  

Expression of T-cadherin in hepatocellular carcinoma and its relationship with relapse and metastasis of tumor

作　　者：李东良[1] 张志强[1] 陈少华[2] 张世安[1] 方坚[1] 林小钦[3] 张霞[1] 江艺[2] 

机构地区：[1]南京军区福州总医院肝胆内科,福州350025 [2]南京军区福州总医院肝胆外科,福州350025 [3]福建医科大学孟超肝胆医院,福州350108

出　　处：《解放军医学杂志》2015年第4期315-318,共4页Medical Journal of Chinese People's Liberation Army

基　　金：福建省自然科学基金(2012J01399);福州市科技计划项目(2012-S-157-2)~~

摘　　要：目的探讨T-钙黏附蛋白(T-cadherin)在肝细胞癌中的表达及其与肿瘤侵袭转移的关系。方法收集2010年5月-2011年10月于南京军区福州总医院行手术切除的肝癌患者的新鲜癌组织和癌旁2cm以上肝组织标本,采用免疫组织化学方法观察不同恶性生物学程度以及手术前TNM分期相同,术后1年内出现或未出现复发和(或)转移的HCC患者的癌组织及癌旁组织中T-cadherin基因的表达;采用RT-PCR方法检测T-cadherin基因mRNA的表达情况。结果肝癌组织中T-cadherin的异常表达率高达46.7%(28/60),明显高于癌旁肝组织6.7%(4/60),两组相比差异具有统计学意义(χ2=24.55,P=0.000)。在高分化与中低分化的HCC患者癌组织中T-cadherin的异常表达率分别为30.0%(6/20)和35.0%(7/20),两者比较差异无统计学意义(χ2=0.114,P=0.736)。在出现转移的HCC患者癌组织中T-cadherin的异常表达率[75%(15/20)]明显升高,与未出现转移的HCC患者[32.5%(13/40)]相比,差异具有统计学意义(χ2=9.676,P=0.002)。T-cadherin mRNA检测结果显示癌组织T-cadherin表达缺失(高度异常表达),癌旁组织在多数患者中呈正常表达(T-cadherin阳性)。结论 T-cadherin基因在HCC中异常表达,且与肝细胞癌的复发转移密切相关,有可能作为判断HCC预后的预测指标和药物研究靶点。Objective To investigate the expression of T-cadherin in hepatocellular carcinoma and its relationship with tumor relapse and metastasis. Methods The gene expressions of T-cadherin in hepatocelular carcinoma tissue with different malignancy grades and its matched adjacent tissues were assessed by immunohistochemistry. The samples were obtained from patients both with relapse and/or metastasis, without relapse, and without metastasis within one year post-operation, and they were in the same TNM stage before operation. The mRNA expression of T-cadherin in hepatocellular carcinoma and matched adjacent tissues was assessed by RT-PCR. Results The abnormal expression rate of T-cadherin in hepatocelular carcinoma was 46.67%（28/60）, which was significantly higher than that of the adjacent tissue（6.67%, 4/60, χ2=24.55, P=0.000）. No difference was observed in the expression of T-cadherin between wel-differentiated and poorly differentiated hepatocelular carcinoma tissues（30% vs 35%, χ2=0.114, P=0.736）. The abnormal expression rate of T-cadherin in hepatocelular carcinoma in the patients with metastasis elevated to 75%（15/20）, while it was remarkably lower in 32.5%（13/40） of the patients who had no metastasis（χ2=9.676, P=0.002）. The RT-PCR results showed that the mRNA expression of T-cadherin in hepatocelular carcinoma significantly decreased compared with that of its adjacent tissues. Conclusions There is an abnormal expression of T-cadherin in hepatocelular carcinoma, and it is found to be closely correlated to the tumor recurrence and metastasis. Abnormal expression of T-cadherin in hepatocellular carcinoma may be used as a prognostic indicator and drug research target.

关 键 词：癌 肝细胞 钙黏着糖蛋白类 复发 肿瘤转移 

分 类 号：R735.7[医药卫生—肿瘤]