结膜松弛症患者结膜成纤维细胞中穿透素3和肿瘤坏死因子-α刺激基因6的表达  

Expressions of pentraxin-3 and tumor necrosis factor-α stimulated gene-6 proteins in fibroblasts of conjunctivochalasis patients

作　　者：韩竹梅[1] 张振永[1] 张兴儒[1] 柯梅青 李青松[1] 项敏泓[1] 

机构地区：[1]上海中医药大学附属普陀医院眼科,200062

出　　处：《中华实验眼科杂志》2015年第5期436-439,共4页Chinese Journal Of Experimental Ophthalmology

基　　金：上海市教委计划内项目（2011JW63）;上海市卫生局科研基金项目（A-175）

摘　　要：背景 结膜松弛症是老年人中常见的眼表疾病,目前发病机制尚未明确,研究表明结膜松弛症常伴有局部炎症反应,炎性细胞因子在其发生和发展过程中发挥一定的作用. 目的 探讨穿透素3（PTX-3）和肿瘤坏死因子-α刺激基因6（TSG-6）在结膜松弛症患者结膜成纤维细胞中的表达. 方法 纳入2011年1月至2012年6月在上海中医药大学附属普陀医院确诊的结膜松弛症患者13例13眼、年龄相关性白内障患者16例16眼及翼状胬肉患者15例16眼,在相应的手术治疗过程中收集结膜组织标本进行成纤维细胞的原代培养和传代.选取3～6代对数生长期细胞用于实验,采用（ELISA）法检测并比较3种细胞培养液上清中PTX-3和TSG-6蛋白的质量浓度.结果 倒置显微镜下观察组织块透亮性差,呈暗黑色区域,周围有细胞溢出,传至第3代,为形态一致的纤维样细胞,大小均一,呈放射状排列,长梭形或两头尖的长条状,细胞质中有一个卵圆形的细胞核,周围有长短不等的细胞突起相互交联.结膜松弛症组患者成纤维细胞培养液上清中PTX-3的质量浓度为（2 182.33±738.68） pg/ml,明显高于年龄相关性白内障组的（738.32±335.00） pg/ml和翼状胬肉组的（981.37±416.04） pg/ml,3个组间总体差异有统计学意义（F=6.474,P=0.032）;结膜松弛症组、翼状胬肉组和年龄相关性白内障组患者成纤维细胞培养液上清中的TSG-6的质量浓度分别为（59.10±6.52）、（53.99±11.16）和（35.01 ±5.33） pg/ml,3个组间总体比较差异有统计学意义（F=7.421,P=0.024）,其中年龄相关性白内障组患者成纤维细胞培养液上清中TSG-6的质量浓度明显低于结膜松弛症组和翼状胬肉组,差异均有统计学意义（均P＜0.05）,但结膜松弛症组与翼状胬肉组间TSG-6的质量浓度差异无统计学意义（P＞0.05）.结论 炎症反应参与结膜松弛症的发生和发展过程,PTX-3、TSG-6在局部组织中的表Background Conjunctivochalasis is an age-related inflammatory ocular surface disease manifesting redundant,loose conjunctiva folds.Studies showed that conjunctivochalasis often accompanies with inflammatory response,indicating that a variety of inflammatory cytokines are involved in pathogenesis of conjunctivochalasis.Objective This study attempted to investigate the expressions of pentraxin-3 （PTX-3） and tumor necrosis factor-α stimulated gene-6 （TSG-6） proteins in fibroblasts of conjunctivochalasis.Methods The protocol was approved by Ethic Committee of Putuo Hospital affiliated Shanghai University of Traditional Chinese Medicine.Thirteen eyes of 13 patients with conjunctivochalasis,16 eyes of 16 patients with age-related cataract and 16 eyes of 15 patients with pterygium were included in Putuo Hospital Affiliated to Shanghai University of Traditional Medicine from January,2011 to June,2012.The samples of conjunctival tissue were collected in the patients during surgery under the informed consent,and fibroblasts were cultured and passaged by explant culture method.Content of PTX-3 and TSG-6 proteins in cell supernatant was detected by ELISA.Results Translucent poor,dark black area was around the cell overflow.Cultured cells of third generation showed long shuttle type,uniform size,radiated out in all directions,and oval nuclei was sited in the cytoplasm,surrounded by varying the length of cross-linking of cell processes.The concentrations of PTX-3 protein in cell supernatant were （2 182.33 ± 738.68） pg/ml in the conjunctivochalasis group,which were higher than （738.32±335.00） pg/ml in the age-related cataract group and （981.37±416.04）pg/ml in the pterygium group,showing a significant difference among the three groups （F =6.474,P=0.032）.The contents of TSG-6 proteins in cell supernatant were （59.10±6.52） pg/ml,（53.99± 11.16） pg/ml and （35.01±5.33）pg/ml in the conjunctivochalasis group,pterygium group and age-related cataract group,with a significant difference among the

关 键 词：结膜疾病 成纤维细胞 TENON囊 细胞培养 酶联免疫吸附测定法 结膜松弛症 抗炎剂 

分 类 号：R777.3[医药卫生—眼科]