微小RNA-30a靶向Beclin1抑制舌鳞状细胞癌细胞自噬活性的研究  

Beclin1-targeted microRNA-30a inhibits autophagic activity in tongue squamous cell carcinoma cell lines

作　　者：梁建锋[1] 王成[1] 翁军权[1] 陈冠辉[1] 侯劲松[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,广州510055

出　　处：《中华口腔医学研究杂志（电子版）》2015年第2期5-8,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：广东省自然科学基金(10151008901000025);广东省科技计划(2013B021800059);广州市科信局对外科技合作专项(2012J5100008)

摘　　要：目的研究微小RNA(mi RNA)-30a转染对舌鳞状细胞癌细胞自噬相关基因Beclin1表达及其自噬活性的影响,探讨舌鳞状细胞癌细胞自噬活性调控的相关机制。方法利用脂质体转染技术,将内源性mi RNA-30a模拟物和抑制物分别转染至舌鳞状细胞癌UM1、UM2和SCC25细胞。实验设mi RNA-30a mimic组、mi RNA-30a inhibitor组和对照组;通过实时荧光定量聚合酶链反应(PCR)检测转染前后Beclin1基因m RNA表达变化,通过Western blot检测Beclin1、LC3的蛋白表达变化。结果在mi RNA-30a mimic组中,Beclin1和自噬标记蛋白LC3-Ⅱ表达量与对照组相比均明显下降(P<0.05);在mi RNA-30a inhibitor组中,Beclin1和LC3-Ⅱ表达量较对照组均明显上升(P<0.05)。结论 mi RNA-30a负调控Beclin1表达,并抑制舌鳞状细胞癌细胞的自噬水平。Objective To investigate the effect of micro RNA（mi RNA）-30 a on the expression of beclin1 and the autophagic activity in tongue squamous cell carcinoma cell lines. Methods Mi RNA-30 a mimic and inhibitor were transfected into tongue squamous cell line cell UM1, UM2 and SCC25 with the lipofection transfection. Cells were cultured and divided into 3 groups which including mi RNA-30 a mimic group, mi RNA-30 a inhibitor group and control group. The expression of Beclin1 and LC3 was analyzed using real-time quantitative polymerase chain reaction and western blotting. Results Transfection with mi RNA-30 a mimic caused a decrease in the m RNA and protein expression of Beclin1 compared with control group（P〈0.05）. Meanwhile, the expression of LC3-Ⅱ protein was significantly lower than the control group（P〈0.05）; By contrast, treatment with the mi RNA-30 a inhibitor resulted in an increase in beclin1 m RNA and protein（P〈0.05）. The LC3-Ⅱ protein was significantly increased as well in this group（P〈0.05）. Conclusion Mi RNA-30 a plays a suppressive effect on the expression of Beclin1 and autophagic activity in tongue squamous cell carcinoma cell lines.

关 键 词：微小RNA-30a BECLIN1 舌 癌 鳞状细胞 自噬 

分 类 号：R739.8[医药卫生—肿瘤]