高糖状态下人牙龈上皮细胞的生物学特性  

Effects of high glucose on the biological characteristics of human gingival epithelial cells

作　　者：杨熙[1] 谢宝仪[1] 黄馨[1] 轩东英[1] 吴嘉怡[2] 徐喆[3] 章锦才[1] 

机构地区：[1]广东省口腔医院.南方医科大学附属口腔医院牙周科,广州510280 [2]广东省深圳牙科医疗中心牙周科,深圳518001 [3]北京大学深圳医院口腔科,深圳518036

出　　处：《中华口腔医学研究杂志（电子版）》2015年第2期9-12,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：国家自然科学基金(81371151;81271160);广东省自然科学基金博士启动项目(2014A030310405)

摘　　要：目的观察高糖状态对人牙龈上皮细胞生物学特性的影响。方法原代培养人牙龈上皮细胞(h GEC),取第三代细胞分为正常组(培养液含5.5 mmol/L D-葡萄糖)、高糖组(培养液含25 mmol/L D-葡萄糖)和甘露醇组(含5.5 mmol/L D-葡萄糖及19.5 mmol/L甘露醇的培养基),采用MTS法检测细胞的增殖情况,流式细胞术检测细胞的生长周期及凋亡情况,划痕实验检测细胞迁移能力。采用重复测量的方差分析进行统计分析。结果高糖组、正常组、甘露醇组三组h GEC相比较,细胞增殖活性(F=5.053,P=0.052)、细胞周期分布(F=1.252,P=0.351)及凋亡细胞比例(F=0.325,P=0.717)差异均无统计学意义;划痕24 h时,糖尿病组的划痕迁移率显著低于正常组(F=54.453,P=0.000)。结论高糖状态下h GEC增殖及凋亡无显著性改变,但细胞迁移能力出现显著下降。Objective To investigate the biological characteristics of human gingival epithelial cells（ h GECs） in high-glucose environments. Methods Cultured primary h GECs, logarithmic growth phase of the 3rd passage cells were used for subsequent experiments. Experimental groups ：（1） high glucose group： K-SFM medium containing 25 mmol / L D-glucose;（2） normal group： K-SFM medium containing 5.5 mmol / L D-glucose;（3） mannitol group： K-SFM medium containing 5.5 mmol / L Dglucose and 19.5 mmol / L mannitol. We used the MTS method to detect the proliferation of h GECs,applied flow cytometry to detect cell cycle and apoptosis of h GECs, and utilized wound healing test to detect migration of h GECs. Comparisons of the data among different groups were performed by repeated measures analysis of variance（ANOVA）. Results There is no statistically significant difference in cell proliferation activity（F = 5.053,P = 0.052）, proportion of cell proliferate（DNA synthesis phase）（F =1.252,P = 0.351） and cell apoptosis（F = 0.325,P = 0.717）. Wound healing test showed after culture for24 h, the scratch migration rate of h GECs in the high group was significantly lower than cells in the normal glucose group and mannitol group（F = 54.453,P = 0.000）. Conclusion High glucose had no significantly effect on cell morphology, cell proliferation and apoptosis of h GECs. However, it weakened the ability of cell migration of hGECs.

关 键 词：高糖 牙龈上皮细胞 增殖 凋亡 细胞迁移 

分 类 号：R781.42[医药卫生—口腔医学]