血小板源性生长因子与流体剪切力对成骨细胞增殖及c-fos表达的影响  

Effect of platelet-derived growth factors and fluid shear stress on cell proliferation and c-fos expression in osteoblasts

作　　者：陈晓丹[1] 向映辉[1,2] 覃峰[1] 杨志[1] 谭淑仪[1] 宋子珺[1] 付强[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,广州510055 [2]常州市口腔医院牙体牙髓科,213000

出　　处：《中华口腔医学研究杂志（电子版）》2015年第2期22-25,共4页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：广东省科技计划(2013B051000032)

摘　　要：目的研究血小板源性生长因子(PDGF)与流体剪切力(FSS)对成骨细胞增殖及c-fos表达的影响。方法将0、10、30和50 ng/ml的PDGF作用于成骨细胞,CCK-8、实时荧光定量聚合酶链反应(PCR)分别检测细胞增殖和c-fos m RNA表达;将PDGF作用于成骨细胞,同时以12 dyne/cm2的FSS加载1 h,分别采用CCK-8、实时荧光定量PCR和Western blot检测成骨细胞增殖、c-fos m RNA和蛋白的表达。结果 PDGF或FSS单独作用均能促进成骨细胞增殖(FFSS=6.500,PFSS<0.05;FPDGF=6.077,PPDGF<0.05),并使c-fos m RNA表达水平显著升高(FFSS=6.425,PFSS<0.05;FPDGF=7.549,PPDGF<0.05);但PDGF与FSS间不存在协同作用(F增殖=1.826,P增殖>0.05;Fc-fos m RNA=2.101,Pc-fos m RNA>0.05;Fc-fos蛋白=1.561,Pc-fos蛋白>0.05)。结论 PDGF单独作用或与FSS联合应用均可促进成骨细胞增殖和c-fos基因的表达,但PDGF与FSS间不存在协同作用。Objectives To study the effect of platelet-derived growth factor（PDGF） and fluid shear stress（FSS） on cell proliferation and c-fos expression in osteoblasts. Methods Different concerntrations of PDGF（0, 10, 30, 50 ng / ml） were used to stimulate primary cultured osteoblasts, respectively. CCK-8and real-time quantitative PCR were performed to detect cell proliferation and c-fos m RNA expression.Furthermore, osteoblasts were stimulated by PDGF and loaded by FSS of 12 dyne / cm2 for 1 h, together.CCK-8, real-time quantitative PCR and western blot were performed to detect cell proliferation, c-fos m RNA and protein expression, respectively. Results PDGF or FSS can respectively increase cell proliferation（FFSS= 6.500,PFSS 〈0.05;FPDGF= 6.077,PPDGF 〈0.05） and the expression levels of c-fos m RNA in osteoblasts（FFSS= 6.425,PFSS 〈 0.05;FPDGF= 7.549,PPDGF〈 0.05）. There was no synergistic effect between PDGF and FSS（Fproliferation= 1.826,Pproliferation 〉0.05;Fc-fos m RNA= 2.101,Pc-fos m RNA 〉0.05;Fc-fos protein= 1.561,Pc-fos protein〉0.05）. Conclusion PDGF itself or combined with FSS can increase cell proliferation and c-fos expression levels in osteoblasts, but there is no synergistic effect between PDGF and FSS on cell proliferation and c-fos expression in osteoblasts.

关 键 词：血小板源性生长因子 流体剪切力 成骨细胞 C-FOS 增殖 

分 类 号：R78[医药卫生—口腔医学]