中华小型猪心肌梗死后移植同种异体骨髓间充质干细胞归巢时间窗  

Homing time window of allogeneic bone marrow stem cells transplanted into Chinese miniature pigs with myocardial infarction

作　　者：香丽萍 关丽娜[1] 史琪[1] 穆玉明[1] 

机构地区：[1]新疆医科大学第一附属医院心脏超声诊断科,新疆维吾尔自治区乌鲁木齐市830011

出　　处：《中国组织工程研究》2015年第10期1551-1555,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(81271590)~~

摘　　要：背景:心肌梗死后不同时间点进行骨髓间充质干细胞移植,其归巢至损伤组织的能力以及起到的修复作用将有很大的不同。目的:探索心肌梗死后移植的骨髓间充质干细胞归巢于心肌组织的最佳时间窗。方法:将18头中华小型猪采用开胸结扎左冠状动脉前降支的方法建立急性心肌梗死模型。造模成功后1 d,3 d,1周,2周,3周,4周6个时间点经冠状动脉注射Brd U标记的骨髓间充质干细胞,于移植后3 d处死动物,检测心肌梗死区骨髓间充质干细胞的归巢量。结果与结论:心肌梗死后1 d,3 d,1周,2周,3周,4周均可见有细胞核呈棕黄色的Brd U标记的阳性细胞,其中心肌梗死后1周的阳性细胞分布最多且最集中,高于其他5个时间点(P<0.05)。说明骨髓间充质干细胞归巢的最佳时间窗为心肌梗死后1周,在此时间点给予干细胞移植促进心肌修复有一定意义。BACKGROUND： Due to the different transplantation time after myocardial infarction, the homing ability of bone marrow mesenchymal stem cells to damaged tissues as well as the repairing role will be very different.OBJECTIVE： To explore the optimal window time for the homing of bone marrow mesenchymal stem cells to the myocardial tissue after myocardial infarction.METHODS： Eighteen Chinese miniature pigs were modeled by the ligation of left anterior descending coronary artery. Brd U-labeled bone marrow mesenchymal stem cells were injected via the coronary artery at 1 day, 3 days,1 week, 2 weeks, 3 weeks, 4 weeks after modeling, respectively. Then, the animals were killed at 3 days after cell transplantation to detect the home of bone marrow mesenchymal stem cells in the infarct zone.RESULTS AND CONCLUSION： Brd U-labeled positive cells with brown nuclei were visible at 1 day, 3 days, 1 week,2 weeks, 3 weeks, and 4 weeks after myocardial infarction, especially at 1 week after myocardial infarction（P〈0.05）. It indicates that the best homing window for bone marrow mesenchymal stem cells was at week after myocardial infarction, when the stem cell transplantation is given to be able to promote myocardial repair.

关 键 词：骨髓 间质干细胞移植 心肌梗塞 干细胞 移植 归巢 骨髓间充质干细胞 心肌梗死 时间窗 中华小型猪 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]