山慈菇多糖对荷H22肝癌小鼠的抗肿瘤机制研究  

Study on Anti-tumor Effect of Pseudobulbus Cremastrae Seu Pleiones Polysaccharide on Mice with H22 Hepatocellular Carcinoma

作　　者：徐小娟[1,2] 蔡懿鑫 毛宇[1] 徐芳[1] 刘湘新[4] 贺建华[3] 

机构地区：[1]湖南农业大学食品科学技术学院,湖南长沙410128 [2]湖南省医药技工学校,湖南长沙410000 [3]湖南农业大学动物科学技术学院,湖南长沙410128 [4]湖南农业大学动物医学院,湖南长沙410128

出　　处：《食品研究与开发》2015年第7期23-25,共3页Food Research and Development

基　　金：中日政府间国际合作项目(2011DFA30490-2)

摘　　要：研究山慈菇多糖对H22肝癌实体瘤小鼠的抗肿瘤作用及其机制。制作小鼠H22实体瘤肝癌模型,通过脾脏指数、胸腺指数分析其对小鼠免疫器官的影响,Elisa检测血清白介素-2(IL-2)含量、肿瘤坏死因子(TNFα)含量,免疫组化法观察(Bcl-2)表达水平。山慈菇多糖的低、中、高剂量组抑瘤率分别为43.74%、37.57%、30.76%,;能增强血清中IL2、TNFα活性;抗凋亡因子Bcl-2的表达量不同程度地减少。山慈菇多糖对H22肝癌小鼠具有一定的肿瘤抑制作用,本次实验不体现剂量依赖性,显示山慈菇多糖无毒副作用,能增强机体的抗肿瘤能力,降低肿瘤的持续增殖能力。To study on the anti-tumor effect and mechanism of pseudobulbus cremastrae seu pleiones polysaccharide on mice with H22 hepatocellular carcinoma. The solid tumor of H22 were established to observe the anti-tumor effect of pseudobulbus cremastrae seu pleiones polysaccharide. The thymus index and spleen index were used to analyze the influence. The serum interleukin-2 （IL-2） and tumor necrosis factor-alpha （TNF-α） levels were detected by ELISA.SABC Immunohistochemical method to observe the Bcl-2 expression level. The solid tumor inhibition ratio of low, middle and high dose of pseudobulbus cremastrae seu pleiones polysaccharide treated groups were 43.74 %,37.57%,30.76%,respectively, Increases in serum IL2,TNFαactivity, could control the expression of Bcl-2. pseudobulbus cremastrae seu pleiones polysaccharide could inhibit the H22 solid tumor on mice with not a dose-dependent and no obvious side effect. The probable route of inhibitory effect of it on mice with H22 solid tumor is immune-enhancement and acceleration of cell apoptosis.

关 键 词：抗肿瘤 山慈菇多糖 肝癌H22 IL-2 TNFΑ bcl-2 

分 类 号：R285.5[医药卫生—中药学]