α2-f(56-57)：YS的ACE活性抑制作用及在酸奶中的应用  

Inhibition Effect of α_2-f(56-57):YS on ACE Activity and Its Application in Yoghourt

作　　者：孙振刚[1] 武文起 冯永强[1] 胡志和[2] 赵勇[2] 

机构地区：[1]天津海河乳业有限公司,天津300402 [2]天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院,天津300134

出　　处：《食品研究与开发》2015年第8期5-9,共5页Food Research and Development

基　　金：天津市科技支撑项目(14ZCZDNC00017);天津市应用基础与前沿技术研究计划重点项目(14JCZDJC34500)

摘　　要：评价乳源肽α2-f(56-57):YS的血管紧张素转化酶(ACE)活性抑制作用及降压效果,并将其应用于发酵酸奶,制备具有降压作用的发酵酸奶。采用分光光度法检测α2-f(56-57):YS对ACE活性的抑制作用,用不同剂量的α2-f(56-57):YS及添加α2-f(56-57):YS的发酵酸奶灌胃原发性高血压大鼠(SHR),评价其降血压效果。结果表明,片段α2-f(56-57):YS具有很好的ACE活性抑制作用,其IC50值为11.89μg/m L;体内检测表明,用低、中、高(1、3、9 mg/kg mb)剂量灌胃原发性高血压大鼠(SHR),均显示有降血压作用,在灌胃后4 h,其血压分别降低了18.25、31.38和36.88 mm Hg,8 h后恢复到初始血压值。添加α2-f(56-57):YS的发酵酸奶,以10 m L/kg mb的剂量灌胃SHR,在灌胃后4 h,对SHR的收缩压(SBP)降低了32.5mm Hg,8 h后恢复到初始值。因此,α2-f(56-57):YS具有降血压作用,应用于发酵酸奶中,可制备具有降压功能的酸奶。Objective of this research was to evaluate inhibition effect of α2-f（56-57）：YS, which from milkα2-casein, on ACE （angiotensin converting enzyme）activity, and its antihypertensive efficacy. And it was applied to produce yoghourt, which had antihypertensive efficacy. The inhibition effect of α2-f （56-57）：YS on ACE activity was evaluated using spectrophotometric method. The antihypertensive efficacy of α2-f（56-57）：YS and the yoghourt which contain α2-f（56-57）：YS was evaluated by gavage SHR（pontaneously hypertensive rat） in different dose. Results showed thatα2-f（56-57）：YS had good inhibition effect on ACE activity in vitro, and its median inhibitory concentration （IC50） was 11.89μg/mL. SHR was given α2-f （56-57）：YS in different dose （1, 3, 9 mg/kg mb） by gavage, after given 4 hour, the blood pressure of SHR was reduced 18.25, 31.38 and 36.88 mm Hg, respectively, and it was return to the initial blood pressure values after given 8 hour. SHR was given the yoghourt of containing α2-f （56-57）：YS in 10 mL/kg mb by gavage, after given 4 h, its SBP was reduced 32.5 mm Hg, and after given 8 h, the SBP return to the initial value. Therefore,α2-f （56-57）：YS had antihypertensive efficacy, and applied it in yoghurt, could give the yoghourt antihypertensive efficacy.

关 键 词：ACE活性抑制肽 α2f(56-57) YS 发酵酸奶 降血压 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程]