香菇多糖柱前衍生化高效液相色谱指纹图谱研究  

HPLC Fingerprint of Mushroom Polysaccharides by Pre-column Derivatization

作　　者：陈志辉[1] 谭丽容 罗明[1] 黄绮敏 张美玲 魏刚[1] 

机构地区：[1]广州中医药大学,广州510006 [2]无限极(中国)有限公司,江门529156

出　　处：《医药导报》2015年第5期649-654,共6页Herald of Medicine

基　　金：广东省部产学研结合项目(2012B090600025)

摘　　要：目的建立香菇多糖1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生高效液相色谱(HPLC)指纹图谱。方法采用水提醇沉法提取香菇多糖,经三氟乙酸(TFA)水解和PMP柱前衍生后,HPLC法测定香菇多糖的单糖组成。结果在所建立的指纹图谱中,共标示出13个特征共有峰,鉴别主要色谱峰6个,分别是甘露糖、D-葡萄糖醛酸、D-葡萄糖、半乳糖、木糖和L-岩藻糖,其中葡萄糖比例最高,其次是甘露糖、半乳糖及岩藻糖。结论该方法稳定、简便、重复性好,可为香菇的质量控制提供参考。Objective To establish the chromatographic fingerprint of mushroom polysaccharides by 1-phenyl-3-methyl-5-pyrazolone（ PMP） pre-column derivatization. Methods The mushroom polysaccharides was extracted by hot distilled water, precipitated by alcohol, and hydrolyzed into monosaccharides by Trifluoroacetic Acid （TFA）. The hydrolysate was derivatized with 1-phenyl-3-methyl-5-pyrazolone （ PMP ） and tested via HPLC to study the monosaccharide components in mushroom polysaccharides. Results Fingerprint was established with 13 common peaks, 6 peaks in which were identified as mannose, D-glucuronic acid, D-glucose, galactose, xylose and L-fucose. The glucose accounted for the most, followed by mannose, galactose and fucose. Conclusion Development of fingerprint chromatogram by HPLC is a stable, simple, and repeatable way, which can be applied to the quality control of mushroom polysaccharides.

关 键 词：香菇多糖 单糖 柱前衍生 色谱法 高效液相 指纹图谱 

分 类 号：P282.71[天文地球—地图制图学与地理信息工程] R284[天文地球—测绘科学与技术]