牙龈卟啉单胞菌脂多糖对人脐静脉内皮细胞表达趋化因子IL-8和MCP-1的影响  

Effects of Porphyromonas gingivalis lipopolysaccharide on the expressions of IL- 8 and MCP- 1 in HUVECs

作　　者：胡琳琳[1] 林玉祥[1] 肖莉[1] 李霞[1] 贾慧杰[1] 李文军[1] 葛颂[1] 

机构地区：[1]遵义医学院附属口腔医院牙周科,贵州遵义563099

出　　处：《遵义医学院学报》2015年第2期154-159,共6页Journal of Zunyi Medical University

基　　金：国家自然科学基金资助项目(NO:8126016)

摘　　要：目的观察牙龈卟啉单胞菌(P.gingivalis)脂多糖(lipopolysaccharide,LPS)对人脐静脉内皮细胞(HUVECs)产生趋化因子的影响,探讨P.gingivalis在动脉粥样硬化(atherosclerosis,As)病变过程中的可能作用。方法复苏P.gingivalis菌株(ATCC 33277)并于厌氧条件下培养,采用热酚水法提取P.gingivalis-LPS,并加以纯化。应用红外线光谱、鲎实验对提取的脂多糖予以鉴定。体外培养HUVECs并加入不同终浓度的P.gingivalis-LPS,采用酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)检测共同培养2、6、24 h后,HUVECs分泌IL-8和MCP-1的量。结果本研究条件下,应用不同浓度P.gingivalis-LPS刺激HUVECs 2、6、24 h后,细胞表达IL-8和MCP-1蛋白水平升高。结论 P.gingivalis-LPS能上调HUVECs的IL-8和MCP-1表达。Objective To study the effects of lipopolysaccharide （LPS） from Porphyromonas gingivalis on the expressions of IL - 8 and MCP - 1 in human umbilical vein endothelial cells （HUVECs） and discuss the possible role of Porphyromonas gingivalis in the process of atherosclerosis. Methods Anaerobic method was employed for P. gingivalis （ ATCC 33277）. P. gingivalis - LPS was prepared by phenol - water methodsand then purified. P. gingivalis - LPS was assessed for their inductions of IL - 8 and MCP - 1 expressions in HUVECs. Enzyme linked immunosorbent assay was used to determine the protein expressions of IL - 8 and MCP - 1 in the culture superna- tant of HUVECs at different time intervals （2, 6 and 24 h）. Results Under the environments of our experiment, P. gingivalis - LPS increased the levels of IL - 8 and MCP - 1. Conclusion LPS of Porphyromonas gingivalis could stimulate HUVECs to express IL- 8 and MCP- 1.

关 键 词：牙龈卟啉单胞菌脂多糖 人脐静脉内皮细胞 白细胞介素-8 单核细胞趋化蛋白-1 

分 类 号：R781.4[医药卫生—口腔医学]