应用荧光原位杂交技术检测54例胚胎停育患者绒毛染色体数目异常的分析  

Chorionic villus karyotyping in 154 embryo arrest cases with a FISH technique

作　　者：马涛[1] 岳军[1] 林鹤[1] 谢兰[1] 

机构地区：[1]四川省医学科学院.四川省人民医院妇产科,四川成都610072

出　　处：《实用医院临床杂志》2015年第3期42-43,共2页Practical Journal of Clinical Medicine

摘　　要：目的应用荧光原位杂交(fluorescence in situ hybridization,FISH)方法对胚胎停育的绒毛组织进行分析,明确胚胎停育与染色体数目异常的情况。方法选择针对13、16、18、21、22、X、Y染色体特异性荧光探针,应用FISH方法,对154例胚胎停育的绒毛组织中的染色体数目进行分析,并了解胚胎停育患者绒毛染色体数目异常情况及其与患者年龄及流产次数的差异。结果 1154例胚胎停育患者染色体数目异常56例(36.4%),其中,常染色体三体40例(71.4%),多倍体4例(7.1%),45,XO 12例(21.4%)。2绒毛染色体数目异常患者的年龄[(32.14±5.21)岁]与染色体数目正常患者的年龄[(29.49±4.07)岁]比较,差异有统计学意义(u=2.145,P<0.05)。3流产次数2次及以上的染色体数目异常发生率(44.8%)高于流产次数为1次的发生率(25.4%),差异有统计学意义(χ2=6.19,P<0.05)。结论染色体数目异常是胚胎停育的重要发病因素,其中常染色体三体最多见;对2次及以上的流产患者,更应关注是否有染色体异常的发生。Objective To investigate the relationship between embryo arrest and abnormal chorionic villus by using a fluores -cence in situ hybridization（FISH） technique.Methods Chorionic villus karyotyping in 154 embryo arrest cases was conducted by u-sing a FISH technique with specific immuno-fluorescent markers targeting chromosome No .13,16,18,21,22,X and Y.The associations of abnormal chromosome karotype with age of pregnancy and history of abortions were analyzed .Results ①Chromosomal abnormality was identified in 56 cases（36.4%）,in which 40 cases were antosomal triploid （71.4%）,4 cases were multiploid（7.1%） and 12 ca-ses were 45,X0（21.4%）.②Median age of the cases with chromosomal abnormality was （32.14±5.21） years old that was older than cases with normal chromosomes（29.49±4.07 years old）（u =2.145,P〈0.05）.Detection rate of chromosomal abnormality in cases with 2 or more abortions was 44.8% that was significantly higher than that in cases with only 1 abortion （ 25.4%） （χ2=6.19 , P〈0.05 ） .Conclusion Embryo chromosomal abnormality is a major cause of embryo arrest ,in which antosomal triploid is the most com-mon abnormality .It should pay attention to examine whether there is abnormal chromosome in cases with 2 or more abortions .

关 键 词：胚胎停育 荧光原位杂交 染色体异常 

分 类 号：R173[医药卫生—妇幼卫生保健]