从江香猪RYR1基因2个SNP位点的多态性  

Polymorphism of Two SNP Loci in RYR1 Gene of Congjiang Xiang Pig

作　　者：王龙龙[1,2] 冉雪琴[3] 王嘉福[1,2] 

机构地区：[1]贵州大学贵州省农业生物工程研究院,贵州贵阳550025 [2]贵州大学生命科学学院,贵州贵阳550025 [3]贵州大学动物科学学院,贵州贵阳550025

出　　处：《贵州农业科学》2015年第4期153-157,共5页Guizhou Agricultural Sciences

基　　金：国家高技术研究发展计划(863计划)"中国地方猪种优异特色性状的功能基因组学研究"(2013AA102503);贵州省农业科技攻关项目"猪繁殖性状候选基因的分离克隆与功能鉴定"[黔科合NY(2013)073];贵州省科技创新人才团队建设专项"贵州省动物遗传育种与生物技术科技创新人才团队"(黔科合人才团队2009-4006);贵州大学本科教学工程项目"基础兽医学教学团队"(BKJX2012007)

摘　　要：兰尼定受体1(Ryanodine Receptor1,RYR1)基因的n等位基因是导致猪应激综合征的主效基因。为探明主产区从江香猪群体中是否存在n等位基因的污染,采用PCR-RFLP和AS-PCR方法对贵州从江香猪群体中n等位基因的多态性进行了检测。结果表明:从江香猪血液基因组DNA经扩增得到659bp的DNA片段,200头从江香猪的基因型均为纯合NN型,N等位基因频率为100%;对659bp扩增片段克隆测序发现1个新的变异位点(T1922C),导致成熟肽V641A替换。T1922C位点基因型均为TC杂合型,T等位基因和C等位基因频率均为50%。经三维结构预测,V641A替换位于RYR1蛋白SPRY1结构域的上游,T1922C变异对猪的兰尼定受体功能可能没有直接影响。The n allele of ryanodine receptor1 （RYR1）gene is the major gene to result in porcine stress syndrome.The polymorphism of n allele in Congjiang Xiang pig population was detected by PCR-RFLP and AS - PCR methods to probe n allelic contamination in Congjiang Xiang pig population of main producing area.The results showed that DNA fragment with 659 bp is amplified from genome DNA of Congjiang Xiang pig’s blood,and the genotype of 200 Congjiang Xiang pigs is homozygous NN,which indicates that N allele frequency is 100%. One new variation locus （T1922C）is detected in clone sequencing of 659 bp amplification fragment,which results in replacement from Valine to Alanine at 641 locus of RYR1.The genotype with T1922C locus is TC heterozygosis and the frequency of T and C allele is 50% respectively.The prediction of three dimensional structure showed that V641A replacement is in the upstream of SPRY1 structural domain of RYR1 protein and T1922C variation may have no direct effect on function of porcine ryanodine receptor.

关 键 词：从江香猪 应激综合征 兰尼定受体1基因 SPRY1 贵州 

分 类 号：S858.31[农业科学—临床兽医学]