红景天苷对原代培养脂肪变性大鼠肝细胞细胞色素P4502E1蛋白表达的抑制作用  

Inhibitory effect of salidroside on cytochrome P4502E1 protein expression in primary-cultured rat hepatocytes with steatosis

作　　者：田丽[1] 尹玲[1] 关莉莉 戴宁[1] 

机构地区：[1]大连医科大学附属第一医院消化内科,辽宁省大连市116011 [2]大连医科大学附属第一医院消化生理实验室,辽宁省大连市116011

出　　处：《实用肝脏病杂志》2015年第3期270-273,共4页Journal of Practical Hepatology

基　　金：辽宁省教育厅科技攻关计划项目(2008115)

摘　　要：目的研究红景天苷对原代培养大鼠脂肪变性肝细胞细胞色素P4502E1(CYP2E1)蛋白表达的抑制作用。方法体外分离、原代培养SD大鼠肝细胞,选取培养48 h的大鼠肝细胞,加入0.25 mmol/L棕榈酸诱导肝细胞脂肪变性,同步加入150μg/ml的红景天苷继续培养24 h,检测培养肝细胞上清液ALT、AST、丙二醛(MDA)和肝细胞甘油三酯(TG)含量的变化。采用油红O染色观察肝细胞脂滴沉积,采用蛋白质印迹法检测CYP2E1蛋白表达的变化。结果以0.25 mmol/L棕榈酸和150μg/ml红景天苷同步培养细胞24 h,红景天苷干预组细胞培养上清ALT[(11.3±1.0)U/L、AST(3.7±0.5)U/L、MDA(1.7±0.2)nmol/ml和TG(105.7±16.8)μg/mg protein]均显著低于棕榈酸模型组[ALT(13.5±1.2)U/L,P<0.05;AST(5.9±0.7)U/L,P<0.05;MDA(4.2±0.9)nmol/ml,P<0.01;TG(268.3±25.8)μg/mg protein,P<0.01];油红O染色显示红景天苷处理肝细胞脂滴明显减少,蛋白质印迹法显示红景天苷能有效抑制脂肪变性肝细胞CYP2E1蛋白表达的上调。结论红景天苷抑制细胞CYP2E1蛋白表达可能是其防治肝细胞脂肪变性的主要机制之一。Objective To investigate the inhibitory effect of salidroside on cytochrome P4502E1（CYP2E1）protein expression in primary-cultured rat hepatocytes with steatosis. Methods The hepatocytes of SD rats were separated and primary-cultured for 48 h. Hepatocytes were then exposed to 0.25 mmol/L palmitic acid incubation for 24 h in the presence or absence of 150 μg/ml salidroside. ALT,AST and malonaldehyde（MDA） in the culture supernatants and triglyceride（TG） contents in the hepatocytes were tested. Lipid accumulation was estimated by oil red-O staining. CYP2E1 protein expression in the hepatocytes was examined by Western blot. Results When compared to palmitic acid group,salidroside decreased ALT [（13.5±1.2） U/L vs.（11.3±1.0） U/L,P〈0.05],AST [（5.9±0.7） U/L vs.（3.7±0.5） U/L,P〈0.05],MDA[（4.2±0.9） nmol/ml vs.（1.7±0.2） nmol/ml,P〈0.01] in the supernatants and TG content in the hepatocytes [（268.3±25.8） μg/mg protein vs.（105.7±16.8） μg/mg protein,P0.01] induced by palmitic acid;Salidroside attenuated severe accumulation of fat drops in the hepatocytes,and suppressed CYP2E1 protein expression up-regulation in hepatocytes with steatosis. Conclusions Salidroside prevents hepatocyte steatosis via suppression of CYP2 E.

关 键 词：脂肪肝 红景天苷 细胞色素P4502E1 肝细胞 氧应激 

分 类 号：R575.5[医药卫生—消化系统]