ABCG5和ABCG8基因在胆囊胆固醇结石和胆固醇息肉形成中的作用  

Effect of ABCG5 and ABCG8 gene expression on formation of cholesterol gallstone and cholesterol polyp

作　　者：邓量[1] 梁明懿[2] 张昆松[3] 许峰峰[1] 黎文峰[1] 张继红[1] 

机构地区：[1]中山大学附属第一医院东院肝胆外科,广州510080 [2]中山大学附属第一医院妇产科,广州3510080 [3]中山大学附属第一医院胆胰外科,广州3510080

出　　处：《中华肝脏外科手术学电子杂志》2015年第2期57-60,共4页Chinese Journal of Hepatic Surgery(Electronic Edition)

基　　金：广东省自然科学基金(S2012040006299;10451008901005954)

摘　　要：目的探讨ABCG5和ABCG8基因在胆囊胆固醇结石和胆固醇息肉发生、发展中的作用。方法回顾性研究2012年3月至2014年3月在中山大学附属第一医院行胆囊切除术的60例患者临床资料。其中男31例,女29例;平均年龄(49±6)岁。所有患者均签署知情同意书,符合医学伦理学规定。根据病理学诊断结果,将患者分为胆囊胆固醇结石组(结石组)、胆囊胆固醇息肉组(息肉组)及正常胆囊切除对照组(对照组)。采用实时荧光定量PCR检测胆囊上皮细胞ABCG5和ABCG8 m RNA相对表达量。3组患者m RNA相对表达量的比较采用单因素方差分析和LSD-t检验。结果结石组ABCG5和ABCG8 m RNA相对表达量分别为3.3±0.9和6.9±1.5,明显高于对照组的2.4±0.6和4.3±1.5(LSD-t=23.58,16.55;P<0.05)。息肉组ABCG5和ABCG8 m RNA相对表达量分别为2.6±0.7和4.6±1.3,与对照组比较差异无统计学意义(LSD-t=1.18,0.73;P>0.05)。结论 ABCG5和ABCG8基因可能通过不同机制在胆囊胆固醇结石和胆固醇息肉的发生、发展中发挥作用。Objective To investigate ABCG5 and ABCG8 gene in the occurrence and development of cholesterol gallstone and cholesterol polyp. Methods Clinical data of 60 patients who underwent cholecystectomy in the First Affiliated Hospital of Sun Yat-sen University from March 2012 to March 2014 were studied retrospectively. Of the 60 patients, 31 were males and 29 were females with a mean age of（49±6） years old. The informed consents of all patients were obtained and the local ethical committee approval had been received. According to the results of pathological diagnosis, the patients were divided into the cholesterol gallstone group（gallstone group）, the cholesterol polyp group（polyp group） and the routine cholecystectomy group（control group）. The relative expression of ABCG5 and ABCG8 m RNA in gallbladder epithelial cells was determined by real-time fluorescence quantitative polymerase chain reaction（PCR）. The comparison of the relative m RNA expression between three groups were conducted using One-way analysis of variance and LSD-t test. Results The relative expression of ABCG5 and ABCG8 m RNA was 3.3±0.9 and 6.9±1.5 respectively in gallstone group, which were significantly higher than 2.4±0.6 and 4.3±1.5 in control group（LSD-t=23.58, 16.55; P〈0.05）. The relative expression of ABCG5 and ABCG8 m RNA was 2.6±0.7 and 4.6±1.3 respectively in polyp group and no significant difference was observed when comparing with those in control group（LSD-t=1.18, 0.73; P〉0.05）. Conclusion ABCG5 and ABCG8 gene may play a role in the occurrence and development of cholesterol gallstone and cholesterol polyp through different mechanisms.

关 键 词：胆囊结石病 息肉 胆固醇 ABCG5 ABCG8 基因表达 

分 类 号：R657.4[医药卫生—外科学]