^(13)C CP/MAS NMR光谱法分析烟草果胶的含量和结构  

Quantitative and structure analysis of pectin in tobacco by ^(13)C CP / MAS NMR spectroscopy

作　　者：刘百战[1] 郑赛晶[1] 高芸[2] 朱晓兰[2] 

机构地区：[1]上海烟草集团有限责任公司烟草行业卷烟烟气重点实验室,上海200082 [2]中国科学技术大学烟草与健康研究中心,合肥230052

出　　处：《中国烟草学报》2015年第2期1-9,共9页Acta Tabacaria Sinica

基　　金：国家烟草专卖局烟草行业卷烟烟气重点实验室开放基金项目(SZBCW201300708)

摘　　要：采用CP/MAS^(13)C NMR光谱法分析烟草果胶的含量和结构。对烟草果胶进行了优化提取后,再对其^(13)C NMR光谱图C-6吸收峰区域进行了详细研究,用于计算果胶中半乳糖醛酸(Gal A)、甲酯度(DM)和乙酰度(DA)的含量,并就建立的定量方法与经典的色谱法和重量法进行了比较。结果表明,CP/MAS ^(13)C NMR光谱法测定烟草样品中果胶的含量是可靠的,化学位移和峰面积可用来计算烟草果胶分子的组成、甲酯度和乙酰度,同步实现烟草中果胶的含量和结构分析。A method utilizing ^13C CP / MAS NMR spectra was developed for quantitative determination and structure analysis of pectin in tobacco. NMR spectra was employed to measure pectin after the extraction of tobacco samples with acid under optimized condition and was applied to calculate galacturonic acid content （GalA）, degree of methylation （DM） and acetytation （DA） by peak fitting in C-6 region. Results were in good agreement with convenient methods （chromography and gravimetric method） and NMR method could realize quantitative and structure analysis of pectin in tobacco simultaneously.

关 键 词：烟草 果胶 NMR光谱 结构分析 

分 类 号：S572[农业科学—烟草工业]