尿中2,5-己二酮测定的顶空固相微萃取气相色谱法  

Headspace solid-phase microextraction-gas chromatography for determination of 2,5-hexanedione in urine

作　　者：邹薇[1] 张梦萍[1] 卫海燕[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院,济南250062

出　　处：《中华劳动卫生职业病杂志》2015年第4期297-298,共2页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：山东省自然基金资助项目（ZR2011HL036）

摘　　要：目的 建立测定尿中2,5-己二酮的顶空固相微萃取气相色谱法.方法 尿液经固相微萃取头萃取后,用气相色谱进行测定,外标法定量.结果 尿中2,5-己二酮浓度在0.1～20.0 μg/ml范围内呈线性,回归方程y=261.36x-1.903 3,r=0.999 2,最低检出浓度为0.01 μg/ml.方法回收率为92.6％～97.1％,相对标准偏差（RSD）为3.3％～5.8％.日内、日间RSD分别为3.8％～6.2％和4.7％～6.3％.结论 该方法无须有机溶剂,操作简单、快速,检测的灵敏度较高,适用于尿中2,5-己二酮的检测.Objective To establish a method for determination of 2,5-hexanedione in urine by headspace solid-phase microextraction-gas chromatography.Methods After extraction by solid-phase microextraction head,2,5-hexanedione in urine was determined by gas chromatography and was quantified by external standard method.Results The concentration of 2,5-hexanedione in urine showed a linear relationship within the range of 0.1-20.0 μg/ml.The regression equation was y=261.36x-1.903 3,r=0.999 2.The minimum detectable concentration was 0.01 μg/ml.The recovery rate was 92.6%～97.1%,with a relative standard deviation （RSD） of 3.3%～5.8%.The intra-day and inter-day RSDs were 3.8%～6.2% and 4.7%～6.3% respectively.Conclusion This determination method has no requirement for organic solvents,features simple and rapid operation,possesses higher detection sensitivity,and applies well to the determination of 2,5-hexanedione in urine.

关 键 词：2 5-己二酮 尿 顶空固相微萃取 色谱法 气相 

分 类 号：R446.12[医药卫生—诊断学] O657.71[医药卫生—临床医学]