microRNA-205对人喉癌细胞株Hep-2细胞增殖的影响  

Effect of microRNA-205on proliferation of laryngeal carcinoma cell line Hep-2

作　　者：李笑天[1] 李巍[1] 姜学钧[1] 

机构地区：[1]中国医科大学附属第一医院耳鼻咽喉头颈外科,沈阳110001

出　　处：《临床耳鼻咽喉头颈外科杂志》2015年第9期831-834,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金青年基金项目(No:81102057)资助

摘　　要：目的:探讨microRNA-205(miRNA-205)对人喉癌细胞株Hep-2细胞增殖的影响。方法:实时定量PCR方法检测27例喉癌组织及癌旁正常组织miRNA-205的表达水平,Western blot方法检测喉癌组织及癌旁正常组织PTEN蛋白表达;将miRNA-205inhibitors及miRNA-205mimics转染至Hep-2细胞,Western blot方法检测转染后PTEN蛋白表达的变化,并通过CCK-8法测定Hep-2细胞增殖能力的变化。结果:miRNA-205在喉癌组织中的表达量较癌旁组织明显升高(P<0.01),PTEN蛋白在喉癌组织中较癌旁组织表达明显降低(P<0.01);转染miRNA-205inhibitor的细胞增殖能力与对照组相比显著降低,细胞内PTEN蛋白表达亦明显升高(P<0.01),转染miRNA-205mimics的细胞增殖能力与对照组相比显著升高,细胞内PTEN蛋白水平明显降低(P<0.01)。结论:miRNA-205可能通过调节PTEN的表达促进喉癌细胞Hep-2的增殖。Objective：To study the effect of microRNA-205（miRNA-205） on proliferation of laryngeal carcinoma cell line Hep-2. Method.. The expressions of miRNA-205 in 27 cases laryngeal carcinoma tissues and adjacent normal tissues were detected by Real-time quantitative PCR, the expression of PTEN protein was detected by Western blot. The expressions of PTEN were detected by Western blot after miRNA-205 inhibitor or miRNA-205 mimics was transfected into Hep-2 cells and Hep-2 cells proliferation was measured by CCK-8 kit. Result.. The expression level of miRNA-205 was significantly higher in laryngeal carcinoma tissues than in adjacent normal tissues （P〈0. 01）, and the expression of PTEN protein was lower in laryngeal carcinoma tissues than in adjacent normal tissues （P〈0.01）. The proliferation rate of Hep-2 cells was decreased significantly and the expression of PTEN protein in Hep-2 cells was increased significantly after miRNA-205 inhibitor was transfected into （P〈0.01）, and the proliferation rate of Hep-2 cells was increased significantly and the expression of PTEN protein in Hep-2 cells was decreased significantly after miRNA-205 mimics was transfected into （P〈0.01）. Conclusion.. miRNA-205 might promote the proliferation of Hep-2 cells by regulating the expression of PTEN.

关 键 词：喉肿瘤 microRNA-205 PTEN HEP-2细胞 

分 类 号：R363[医药卫生—病理学]