CD44在鼻咽癌侧群细胞中的表达及意义  

Expression and its significance of CD44in SP cells of nasopharyngeal carcinoma

作　　者：熊姗姗[1] 邱前辉[2] 詹建东[2] 苏小妹[2] 许咪咪[2] 罗小宁[2] 

机构地区：[1]南方医科大学在读硕士研究生,广州510515 [2]广东省人民医院(广东省医学科学院)耳鼻咽喉头颈外科

出　　处：《临床耳鼻咽喉头颈外科杂志》2015年第9期841-844,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：广东省科技计划项目(No:20120314)

摘　　要：目的:探讨CD44在鼻咽癌侧群细胞(SP细胞)中的表达状况及意义。方法:采用流式细胞仪对培养的鼻咽癌CNE-2细胞进行分选以获得出CD44-SP细胞及CD44+SP细胞,通过细胞迁移实验、平板克隆形成实验、细胞周期分析及对化疗药物的敏感性等实验,统计分析CNE-2、CNE-2SP、CNE-2NSP、CNE-2CD44+SP、CNE-2CD44-SP各细胞之间的生物学差异。结果:鼻咽癌CNE-2细胞中可以提取到2.3%的SP细胞,其中36.5%为CD44+SP细胞。CD44+SP细胞的增殖能力、细胞迁移能力、平板克隆形成能力均显著高于其他细胞(P<0.01),对化疗药物的耐受性也显著高于其他细胞(P<0.01)。结论:SP细胞在鼻咽癌细胞中所占的数量少,但在细胞的增殖方面具有较强活跃性,其具有肿瘤细胞"种子"特性。由于CD44+SP细胞在鼻咽癌增殖生长以及化疗耐药方面起着重要作用,提示CD44可作为鼻咽癌SP细胞表面的标志物之一。Objective：Discussion of expression and its significance of CD44 in SP cells of nasopharyngeal carcinoma. Method ： Flow cytometry was used to sort cultured CNE-2 cells of nasopharyngeal carcinoma for obtaining CD44- SP and CD44＋ SP cells. Biological differences of CNE-2, CNE-2 SP, CNE-2 NSP, CNE-2 CD44＋ SP and CNE-2 CD44-SP cells were statistically analyzed by experiments such as cell migration experiments, plate clone formation assay, cell cycle analysis and sensitivity tests to chemotherapeutics. Result： Two point 3 perent of SP cells were extracted from CNE-2 cells of nasopharyngeal carcinoma, among which 36.5% was CD44＋SP cells. A- bilities of proliferation, cell migration and plate clone of CD44＋SP cells were significantly higher than other cells （P〈0.01）, and its tolerance to chemotherapeutics was significantly higher too （P〈0.01）. Conclusion.. The pro- portion of SP cells in nasopharyngeal carcinoma ceils was small, but SP cells had strong activeness in the aspect of cell proliferation with a ＂seed＂ characteristic of tumor cells. As CD44＋SP ceils played an important role in prolif- eration and chemotherapy resistance of nasopharyngeal carcinoma, it indicated that CD44 can be one of the surface markers of SP cells of nasopharyngeal carcinoma.

关 键 词：鼻咽癌 侧群细胞 CD44 肿瘤标志物 

分 类 号：R739.6[医药卫生—肿瘤]