MicroRNA-493抑制乳腺癌MCF7细胞增殖、迁移和成瘤能力  

MicroRNA-493 Inhibited Proliferation, Migration and Tumor Formation of Breast Cancer Cell MCF7

作　　者：韦唯[1] 葛葵葵[1,2] 徐宇强[1] 黄晋江 黄青山[1,2] 

机构地区：[1]复旦大学生命科学学院遗传学国家重点实验室,上海200433 [2]上海高科联合生物技术研发有限公司,上海201206

出　　处：《中国细胞生物学学报》2015年第4期486-492,共7页Chinese Journal of Cell Biology

基　　金：国家重大新药创制专项(批准号:2013ZX09102057);国家基础科学人才培养基金项目资助的课题~~

摘　　要：为了研究miR-493对乳腺癌MCF7细胞增殖、迁移和肿瘤形成能力的影响及其可能的机制,该文使用pc DNA3.1(+)/miR-493重组质粒转染MCF7细胞,通过筛选获得高表达miR-493的细胞克隆。通过荧光定量PCR检测miR-493在MCF10A和MCF7及各细胞克隆中的表达;CCK8实验检测miR-493对MCF7细胞增殖能力的影响;Transwell和划痕实验观察miR-493高表达后MCF7细胞迁移和侵袭能力的改变,软琼脂克隆形成实验以及裸鼠实验检测miR-493对MCF7细胞肿瘤形成能力的影响。软件预测miR-493可能的靶基因,并通过荧光定量PCR和Western blot进行验证。该研究证明,miR-493能够有效抑制乳腺癌MCF7细胞的增殖、迁移、浸润和肿瘤形成的能力。To research the effect of miR-493 on cell proliferation, migration and tumor formation of breast cancer MCF7 cells, MCF7 cells were infected with recombinant plasmid pc DNA3.1（＋）/miR-493 and cell lines with stably highly expressed miR-493 were selected by G418 screening. The expression of miR-493 in MCF10 A, MCF7 cells and MCF7 clones after infection was detected by fluorescence quantitative Real-time PCR. In order to detect the influence of increased miR-493 expression in MCF7 cells, the proliferation ability was determined using CCK8 assay. The migration ability of MCF7 cells was assessed by Transwell and wound-healing assays. The invasion ability of MCF7 cells was detected by Transwell assay. The impact of miR-493 expression on the tumor formation ability of MCF7 cells was detected by soft agar assay in vitro and nude mice assay in vivo. The target gene was predicted by miRanda and Target Scan softwares and investigated by fluorescence quantitative Real-time PCR and Western blot. The result of CCK8 assay showed that over-expression of miR-493 could inhibit cell proliferation of MCF7 cells. The results of Transwell and wound-healing assays indicated that the abilities of migration and invasion of MCF7 cells were suppressed when miR-493 was up-regulated. And the results of soft agar and nude mice assays also revealed that over-expression of miR-493 could inhibit both clone formation and tumor formation of MCF7 cells.

关 键 词：miR-493 MCF7 细胞增殖 细胞迁移 肿瘤形成 

分 类 号：R737.9[医药卫生—肿瘤]