Ajuba与Twist相互作用调控N-cadherin表达对结直肠癌细胞迁移的影响  

Effects of regulation of N-cadherin expression by interaction of Ajuba and Twist on migration ability of colorectal cancer cells

作　　者：陈宁[1,2] 宋立伟[3] 袁晓圆 王家敏[1] 侯照远[1] 

机构地区：[1]上海交通大学基础医学院生物化学与分子生物学系,上海200025 [2]上海交通大学医学院检验系,上海200025 [3]上海交通大学医学院附属第九人民医院普外科,上海200011

出　　处：《上海交通大学学报（医学版）》2015年第4期465-469,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81172028;81372309;81402177);上海市科委重点项目基金(13JC1401302)~~

摘　　要：目的探索结直肠癌细胞中Ajuba与Twist之间的相互作用,及该作用对于结直肠癌迁移所产生的影响。方法运用免疫共沉淀方法验证外源性Ajuba与Twist蛋白之间是否存在相互作用;荧光素酶活性检测Ajuba对于Twist下游靶基因N-cadherin启动子的转录活性;构建结直肠癌SW1116-sh Ajuba稳转细胞株,并进行Transwell侵袭实验,观察细胞迁移能力变化。结果 Ajuba与Twsit之间存在相互作用,并且Ajuba能通过Twist促进N-cadherin的表达调控。在结直肠癌细胞SW1116-sh Ajuba稳转细胞中可发现,Ajuba基因表达水平降低,蛋白表达下降,能抑制细胞迁移能力。结论 Ajuba是转录因子Twist的共活化因子,能够促进N-cadherin表达,增强结直肠癌细胞的迁移能力。Objective To explore the interaction between LIM protein Ajuba and transcription factor Twist in colorectal cancer cells and the effects of this interaction on the migration ability of colorectal cancer cells. Methods Co-immunoprecipitation test was used to verify whether the interaction exists between exogenous Ajuba and Twist. The transcription activity of Ajuba towards N-cadherin promoter of downstream target gene of Twist was detected by the luciferase activity assay. Ajuba knock-down stable cell lines SW1116-shAjuba were established. Changes of the cell migration ability were detected by the Transwell assay. Results The interaction between Ajuba and Twist existed and Ajuba could enhance the regulation of N-cadherin expression through Twist. The expression of Ajuba gene in SW1116-shAjuba cells decreased; protein expression down- regulated; and cell migration ability was inhibited. Conclusion Ajuba is a new coactivator of transcription factor Twist. Ajuba can promote the expression of N-cadherin and enhance the migration ability of colorectal cancer cells.

关 键 词：结直肠癌 Ajuba TWIST N-CADHERIN 细胞迁移 

分 类 号：R735.34[医药卫生—肿瘤]