长期使用糖皮质激素对大鼠的骨生物力学影响  

The effect of long-term use of glucocorticoids on bone biomechanics of rats

作　　者：孙晓晖[1] 陈文双[2] 陈春玲[2] 吴天秀[3] 

机构地区：[1]广东医学院护理学院,广东东莞523000 [2]广东医学院天然药物研究与开发重点实验室,广东湛江524023 [3]广东医学院基础医学院,广东湛江524023

出　　处：《中国骨质疏松杂志》2015年第4期418-420,451,共4页Chinese Journal of Osteoporosis

基　　金：广东省科技计划项目(2010B031600291;2011B031800229)

摘　　要：目的探讨长期使用糖皮质激素对大鼠的骨质量影响。方法 3月龄SD大鼠共38只,雌性。随机分成4组,分别是:(1)基础对照组(B组,8只,实验开始时处死,建立本批大鼠底线);(2)正常对照组(C组,10只,腹腔注射生理盐水75 mg/kg);(3)低剂量激素组(L组,10只,腹腔注射甲强龙50 mg/kg);(4)高剂量激素组(H组,10只,腹腔注射甲强龙75 mg/kg)。实验开始时,低剂量激素组和高剂量激素组连续使用甲强龙3天(75 mg/kg·d),停用一周后,按计划使用甲强龙,每周一次,用药24周后取股骨和第三腰椎行骨生物力学实验。结果 (1)与正常对照组相比,低剂量激素组和高剂量激素组的实验后体重明显减少(P<0.05);(2)与正常对照组相比,低剂量激素组和高剂量激素组股骨的结构力学参数(载荷、刚性)和材料力学参数(破断强度、刚性模量)均显著降低(P<0.05);(3)与正常对照组相比,低剂量激素组和高剂量激素组腰椎的材料力学参数(破断强度、刚性模量)均显著降低(P<0.05)。结论长期使用糖皮质激素激素,无论剂量高低,均会导致大鼠股骨和腰椎骨质量下降。Objective To determine the effect of long-term use of methylprednisolone on bone biomechanics of rats.Methods Thirty-eight 3-month-old female SD rats were chosen.They were randomly divided into basal control group （ B group, 8 rats were sacrificed at the beginning of the experiment as baseline ） , normal control group （ C group, 10 rats were given intraperitoneal injection of 75mg/kg normal saline）, low dose methylprednisolone group（L group, 10 rats were given intraperitoneal injection of 50 mg/kg methylprednisolone） , and high dose methylprednisolone groug （ H group, 10 rats were given intraperitoneal injection of 75 mg/kg methylprednisolone） .Methylprednisolone was given in L group and H group for 3 days, and stopped for a week.Then the intraperitoneal injection was given once a week according to the plan.After 24 weeks of the treatment, the femurs and the lumbar spine L3 were collected for biomechanical experiment.Results （1） Compared to that in C group, the body weight in L group and H group decreased obviously after the experiment （P〈0.05）.（2） Compared to those in C group, structural mechanical parameters （ load and rigidity） and material mechanical parameters （ breaking strength and modulus of rigidity） of the femurs in L group and H group significantly decreased （P〈0.05）.（3） Compared to those in C group, structural mechanical parameters （load and rigidity） and material mechanical parameters （ breaking strength and modulus of rigidity） of the L3 in L group and H group significantly decreased （ P 〈0.05 ） .Conclusion The long-term use of methylprednisolone, regardless of dose, results in the decrease of bone quality of the femur and the lumbar spine of rats.

关 键 词：糖皮质激素性骨质疏松 大鼠 骨生物力学 

分 类 号：R24[医药卫生—中医临床基础]