F10基因过表达及沉默对绒癌细胞系JEG-3侵袭性的影响  

Influence of F10 gene depletion or over-expression on the invasiveness of choriocarcinoma cell line JEG-3

作　　者：苏晓华[1] 庞战军[1] 

机构地区：[1]南方医科大学南方医院妇产科,广州510515

出　　处：《中华实用诊断与治疗杂志》2015年第5期444-447,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金(81170595)

摘　　要：目的探讨F10基因过表达及沉默对绒癌细胞系JEG-3侵袭相关蛋白酶表达的影响。方法采用细胞转染技术及RNA干扰技术,分别建立和筛选出F10基因稳定过表达及沉默的绒癌细胞系JEG-3。将SPF级裸鼠（4~5周龄）30只随机分为F10过表达组、F10沉默组和未处理组各10只,分别接种F10基因过表达的JEG-3细胞、F10基因沉默的JEG-3细胞和未处理的JEG-3细胞。于接种5周后处死裸鼠取皮下肿瘤,采用免疫组织化学法和Western blot法检测各组瘤体组织中基质金属蛋白酶（matrix metalloproteinase,MMP）、基质金属蛋白酶组织抑制物-1（tissue inhibitor of matrix metalloproteinase,TIMP-1）,血浆纤溶酶原激活物抑制因子（plasminogen activator inhibitor-1,PAI-1）等侵袭相关蛋白酶的表达,并进行比较。结果免疫组织化学结果显示,F10过表达组成瘤组织中MMP-2、MMP-8、MMP-11、MMP-16、MMP-19蛋白表达水平（0.332±0.037、0.473±0.089、0.441±0.031、0.470±0.040、0.548±0.062）高于未处理组（0.209±0.021、0.314±0.108、0.207±0.025、0.400±0.057、0.466±0.038）和F10沉默组（0.049±0.013、0.168±0.034、0.149±0.024、0.240±0.024、0.175±0.029）（P〈0.05）,未处理组高于F10沉默组（P〈0.05）;F10过表达组TIMP-1（0.191±0.027）和PAI-1蛋白（0.130±0.020）表达水平低于未处理组（0.249±0.036、0.284±0.020）和F10沉默组（0.310±0.018、0.463±0.115）（P〈0.05）,未处理组低于F10沉默组（P〈0.05）。wesrern blot检测结果显示,上述侵袭相关蛋白酶在3组间比较差异均有统计学意义（P〈0.05）。结论 F10通过上调MMP-2、MMP-8、MMP-11、MMP-16、MMP-19表达,下调TIMP-1、PAI-1表达,促进绒癌细胞增殖、侵袭和转移。Objective To investigate the influence of F10 gene depletion or over-expression on the expression of invasionrelated proteinase in choriocarcinoma cell line JEG-3.Methods Stable F10 gene over-expression or F10gene-silenced JEG-3choriocarcinoma cell lines were established by cell transfection and RNA interference technology.Thirty SPF nude mice aged from 4to 5 weeks were randomly divided into F10over-expression group（by injecting of F10 gene overexpressed JEG-3cells）,F10gene-silenced group（by injecting of F10gene-silenced JEG-3cells）and control group（by injecting of non-treated JEG-3cells）,with 10 mice in each group.The mice were sacrificed and the subcutaneous tumor tissues were gained 5weeks after the JEG-3cell injection.Western blot and immunohistochemical method were used to detect the expressions of matrix metalloproteinase（MMP）-2,MMP-8,MMP-11,MMP-16,MMP-19,tissue inhibitors of metalloproteinase-1（TIMP-1）,and plasminogen activator inhibitor-1（PAI-1）in subcutaneous tumor tissues in three groups.Results The immunohistochemical method revealed the expressions of MMP-2,MMP-8,MMP-11,MMP-16 and MMP-19 were the highest in F10over-expression group（0.332±0.037,0.473±0.089,0.441±0.031,0.470±0.040,0.548±0.062）,followed by control group（0.209±0.021,0.314±0.108,0.207±0.025,0.400±0.057,0.466±0.038）and F10 silenced group（0.049±0.013,0.168±0.034,0.149±0.024,0.240±0.024,0.175±0.029）in turns（P0.05）.The expressions of TIMP-1and PAI-1 were the lowest in F10over-expression group（0.191±0.027,0.130±0.020）,followed by control group（0.249±0.036,0.284±0.020）and F10 silenced group（0.310±0.018,0.463±0.115）in turns（P0.05）.Western blot showed significant differences in the above invasion-related proteinase indexes among three groups（P0.05）.Conclusion F10 gene could up-regulate the expressions of MMP-2,MMP-8,MMP-11,MMP-16 and MMP-19,and down-regulate the expressions of TIMP-1and PAI-1,which is involved in the proliferation,invasion and metastasis of choriocarc

关 键 词：绒癌 F10基因 基质金属蛋白酶 血浆纤溶醇原激活物抑制因子 JEG-3细胞 

分 类 号：R737.33[医药卫生—肿瘤]