LPS对肝癌细胞株HepG2增殖、凋亡及分泌炎症因子的影响  

Effects of LPS on proliferation,apoptosis and secretion of cytokines of hepatocellular carcinoma cell line HepG2

作　　者：刘亚婷[1] 李涛[1] 徐恩君 周敏[2] 徐元宏[1] 沈继龙[3] 

机构地区：[1]安徽医科大学第一附属医院检验科,合肥230032 [2]安徽医科大学第一附属医院ICU,合肥230032 [3]安徽医科大学第一附属医院安徽人兽共患病重点实验室,合肥230032

出　　处：《安徽医科大学学报》2015年第5期604-607,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30801088;81201488);卫生部应用研究项目"高通量ELISA检测系统化;标准化系列研究"(编号:28-1-50)

摘　　要：目的体外观察不同浓度细菌内毒素脂多糖(LPS)对肝癌HepG2细胞增殖、凋亡以及分泌炎症因子的影响,并探讨可能的机制。方法按照随机数字法将细胞分为对照组及1、10、50、100μg/ml LPS处理组。用MTT检测刺激24、48、72和96 h后细胞的增殖能力;用流式细胞术检测刺激24h后细胞的凋亡情况;用RT-PCR法检测刺激24 h后白介素-6(IL-6)和白介素-8(IL-8)mRNA的表达含量;用化学发光法检测刺激24 h后IL-6和IL-8的表达量,最后用SPSS 19.0对数据进行统计学分析。结果与对照组比较,各处理组刺激24 h和48 h后细胞增殖活性明显升高(P<0.05),而在72、96 h差异无统计学意义(P>0.05);刺激24 h后,1、10、50和100μg/ml LPS处理组以及对照组之间的细胞凋亡率差异无统计学意义(P>0.05);刺激24 h后,与对照组比较,随着刺激浓度的升高,IL-6和IL-8的表达量明显升高(P<0.05)。结论 LPS可以在48 h内促进Hep G2的增殖,对Hep G2细胞的凋亡没有影响,并且LPS作用可以上调IL-6和IL-8水平。Objective To observe the effects of different concentrations of LPS on proliferation, apoptosis and se-cretion of cytokines of liver cancer cell line HepG2 in vitro, and to explore their possible mechanism. Methods The cells were divided into control group and 1, 10, 50, 100 μg / ml LPS treatment groups according to the method of random number. The proliferation of HepG2 cells at post treatment hour （PTH） 24, 48, 72 and 96 （denoted as absorption value） was detected by MTT and the apoptosis of HepG2 cells at PTH 24 was detected by flow cytome-ter. The level of IL-6 and IL-8 mRNA was detected by RT-PCR at PTH 24 and the concentration of IL-6 and IL-8 was detected at PTH 24 by chemiluminescence immunoassay. The data were analyzed with SPSS19. 0. Results Compared with the control group, proliferation of HepG2 cells was significantly increased （P 〈 0. 05） in the treat-ment groups at PTH 24 and 48, but there was no statistically significant difference in proliferation of HepG2 cells at PTH 72 and 96（P 〉 0. 05）. There was no statistically significant difference in apoptosis rate of HepG2 cells at PTH 24 between the groups. Compared with the control group, the expression of IL-6 and IL-8 was significantly in-creased with the increase in concentration of LPS at PTH 24. Conclusion LPS can promote the proliferation of HepG2 cells in 48 hours and has no effect on apoptosis of HepG2 cells. LPS can also up-regulate the expression of IL-6 and IL-8 in HepG2 cells.

关 键 词：脂多糖 HEPG2 细胞 增殖 凋亡 炎症因子 

分 类 号：R73-3[医药卫生—肿瘤]