RNA结合蛋白La对宫颈癌细胞侵袭转移的影响及其机制  

Effects of RNA-binding protein La on migration and invasion of cervical cancer cells

作　　者：陈茜[1] 陈昆仑[2] 刘韡[1] 李牧[1] 高庆[1] 

机构地区：[1]西安交通大学医学部第二附属医院妇产科,陕西西安710004 [2]西安交通大学医学部第二附属医院普通外科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2015年第3期345-348,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助(No.81070536)~~

摘　　要：目的探讨RNA结合蛋白La(La)对宫颈癌细胞侵袭转移的作用及其作用机制。方法采用RNA干扰技术沉默La基因在宫颈癌细胞株HeLa中的表达,并通过G418筛选,构建稳定表达的HeLa的La干扰克隆。采用划痕实验、Transwell小室等检测La对宫颈癌细胞侵袭转移的影响。利用Western blot的方法检测细胞中基质金属蛋白酶2(MMP-2)和基质金属蛋白酶抑制剂2(TIMP-2)的表达,并采用明胶酶谱的方法检测细胞培养上清中MMP-2的活性。结果 La干扰后细胞的迁徙和侵袭能力明显降低,对细胞迁徙距离和穿过Transwell小室的细胞数目进行分析后发现,差异有统计学意义;同时发现在细胞中MMP-2的蛋白表达水平及酶活性均降低,而TIMP-2的蛋白表达水平上调。结论 La对宫颈癌的侵袭转移具有促进作用,这种作用可能与其对基质金属蛋白及其抑制剂的调控相关。Objective To investigate the role of RNA-binding protein La protein in the invasion and migration of cervical cancer cells.Methods RNAi technology was used to silence the La protein in HeLa cell,a cell line of cervical cancer,and then screened by G418.Finally the stably expressed HeLa-shLa cell line was constructed and then wound healing,Transwell,Western blot and gelatin zymography assay were performed. Results After La protein HeLa was silenced by RNAi,the invasion and migration capabilities of HeLa cells were decreased significantly compared with those of the controls.Meanwhile,SiRNA-mediated depletion of La reduced the expression of MMP-2 and increased the expression of TIMP-2.Meanwhile the activity of MMP-2 was reduced too.Conclusion RNA-binding protein La promotes the invasion and migration of cervical cancer cells,which may be related to regulating its matrix metalloproteinases and inhibitors.

关 键 词：RNA结合蛋白 侵袭 宫颈癌 RNA干扰 

分 类 号：R737.33[医药卫生—肿瘤]