磷酸化壳聚糖/无定形磷酸钙复合物与多聚磷酸钠对脱矿牙本质仿生再矿化的影响  

In vitro biomimetic remineralization of dentin collagen by phosphorylated chitosan/amorphous calcium phosphate compound and tripolyphosphate

作　　者：苗瑞婧 张旭[1] 孙迎春[1] 高平[1] 

机构地区：[1]天津医科大学口腔医院,300070

出　　处：《天津医药》2015年第5期518-521,I0003,共5页Tianjin Medical Journal

基　　金：国家自然科学基金青年项目(81200817);天津市应用基础及前沿技术研究计划青年项目(12JCQNJC09200)

摘　　要：目的探讨磷酸化壳聚糖/无定形磷酸钙(P-chi/ACP)与多聚磷酸钠(TPP)对脱矿牙本质体外仿生再矿化的影响,为深龋再矿化治疗提供思路。方法将32颗中度磨耗的离体磨牙制成大小相同的完全脱矿牙本质片32个,其中2个样品用于透射电镜表征,另30个分成2组:P-chi/ACP+TPP组(n=15)经TPP预处理后,固定在放置有P-chi/ACP的体外模型上;P-chi/ACP组(n=15)不经过TPP处理。所有样品根据是否与P-chi/ACP接触分为实验侧与对照侧。处理1周后,进行显微计算机断层扫描定量分析密度变化、透射电子显微镜定性分析微观形态变化。结果显微计算机断层扫描结果显示P-chi/ACP+TPP组的实验侧灰度值高于对照侧(125.42±12.16 vs 96.96±10.56,t=7.124,P<0.01),P-chi/ACP组中的实验侧灰度值高于对照侧(119.39±8.64 vs 105.27±9.42,t=5.329,P<0.01)。透射电子显微镜显示P-chi/ACP+TPP组有纤维内横纹样结构出现,提示脱矿牙本质片实现了其主要成分Ⅰ型胶原的分级化纤维内矿化;P-chi/ACP组未见横纹样结构。结论单独利用P-chi/ACP能够提高脱矿牙本质的密度,但共同利用TPP与P-chi/ACP复合物才能实现完全脱矿牙本质的仿生再矿化。Objective To investigate the remineralizing therapy of deep caries and in vitro biomimetic remineralization of demineralized dentin by phosphorylated chitosan/amorphons calcium phosphate compound （P-chi/ACP） and tripolyphosphate （TPP）. Methods Thirty-two extracted human molars were cut and completely demineralized. Two samples were used to show the characteristics by transmission electron microscopy （TEM）. The other 30 samplea were divided into two groups： fifteen samples were treated by P-chi/ACP and TPP （P-chi/ACP＋TPP group）, the other fifteen samples were not treated by TPP （P-chi/ACP group）. All of the samples were distinguished into experimental side and control side, and then they were set on the in vitro model for 1 week. Micro-computed tomography （μCT） and TEM were used to assess the effects of reminer- alization. Results p.CT detection revealed that the mineral density were higher in the experimental sides （125.42 ± 12.16 and 119.39±8.64） than that of control sides （96.96±10.56 and 105.27±9.42） in both groups （P 〈 0.01）. TEM figures showed that hierarchical intrafibrillar remineralization was realized in samples of P-chi/ACP＋TPP group, while trace amounts of hi- erarchical remineralization was detected in P-chi/ACP group. Conclusion Fully demineralized dentin appears to have the potential to be remineralized with the application of P-chi/ACP. The uhrastructure of samples is better in P-chi/ACP＋TPP group than that of P-chi/ACP group.

关 键 词：牙本质 仿生学 磷酸化壳聚糖 多聚磷酸钠 再矿化 

分 类 号：R781.1[医药卫生—口腔医学] R318.08[医药卫生—临床医学]