浙江南部地区人群白三烯A4水解酶基因突变与缺血性卒中风险的相关性  

Correlation between variants of leukotriene A4 hydrolase gene and risk of ischemic stroke in population of southern Zhejiang Province

作　　者：胡凯凯[1] 池丽芬[2] 易兴阳[2] 邵蓓[1] 

机构地区：[1]浙江省温州医科大学附属第一医院神经内科,325000 [2]温州医科大学附属第三医院神经内科

出　　处：《中华神经科杂志》2015年第5期368-372,共5页Chinese Journal of Neurology

摘　　要：目的 探索浙江南部地区人群白三烯A4水解酶（LTA4H）基因中rs2660845、rs2540493单核苷酸多态性与缺血性卒中的关系.方法 选取2010年9月至2013年6月在温州医科大学附属第三医院神经内科住院的300例脑梗死患者,另选该院同期300名健康体检者作对照.采用PCR和激光解析/电离飞行时间质谱法,分析LTA4H基因中rs2660845、rs2540493位点的单核苷酸多态性.采用完全随机化方法从中随机选取67例脑梗死患者及30名对照者,采用ELISA法检测血清白三烯B4水平.结果 LTA4H基因的rs2660845、rs2540493的突变与卒中风险或Org 10 172急性卒中治疗试验（TOAST）分型均无明显相关性（P＞0.05）.分析血清白三烯B4水平显示,血清白三烯B4浓度在脑梗死组（n=67,0.991±0.305）与对照组（n=30,1.035±0.498）之间差异无统计学意义（P=0.692）,也未发现其在rs2660845位点的各基因型之间差异有统计学意义（AG基因型0.938±0.269,AA基因型1.038±0.268,GG基因型1.043±0.383;P=0.401）.结论 LTA4H基因中rs2660845、rs2540493位点的单核苷酸多态性与浙江南部地区人群的缺血性卒中风险无相关性.Objective To investigate the correlation between two single nucleotide polymorphisms of the leukotriene A4 hydrolase （LTA4H） gene （rs2660845 and rs2540493） and risk of ischemic stroke in population of southern Zhejiang Province.Methods A total of 300 ischemic stroke patients and 300 healthy controls,recruited from the Department of Neurology,Third Affiliated Hospital of Wenzhou Medical University between September 2010 and June 2013,were enrolled in this study.Two single nucleotide polymorphisms of the LTA4H gene （rs2660845 and rs2540493） were analyzed by polymerase chain reaction and matrix-assisted laser desorption/ionization time of flight,respectively.Sixty-seven patients and thirty controls were randomly selected （complete randomization） and detected the serum leukotriene B4 （LTB4）concentration by ELISA method.Results There was no evidence of association between the two variants of LTA4H gene and the risk of ischemic stroke or its TOAST （Trial of Org 10 172 in acute stroke treatment）subtypes （P 〉 0.05）.Analysis of LTB4 levels revealed that there was no statistically significant difference in serum LTB4 concentration between patients （n =67） and controls （n =30; 0.991 ± 0.305 vs 1.035 ± 0.498 ; P =0.692）,and no statistically significant difference in LTB4 concentration was found among the three genotypes of rs2660845 as well （AG genotype vs AA genotype vs GG genotype：0.938 ± 0.269 vs 1.038 ± 0.268 vs 1.043 ± 0.383 ; P =0.401）.Conclusion The present study suggests that there is no association between the two polymorphisms in the LTA4H gene and risk of ischemic stroke in population of southern Zhejiang Province.

关 键 词：脑缺血 卒中 环氧水解酶类 多态性 单核苷酸 突变 

分 类 号：R743.3[医药卫生—神经病学与精神病学]