不规则趋化因子在缺氧缺血性脑损伤新生大鼠脑组织中的表达及参附注射液干预作用的研究  

Study on the expression of CX3CL1 in brain tissue of neonatal rats with hypoxic-ischemic brain damage and the intervention effect of Shenfu injection

作　　者：于乔[1] 王瑞妍[1] 王军[1] 刘芳[1] 张芳芳[1] 

机构地区：[1]徐州医学院附属医院新生儿科,江苏徐州221002

出　　处：《中国妇幼保健》2015年第15期2425-2428,共4页Maternal and Child Health Care of China

基　　金：江苏省卫生厅科技计划重大项目〔H200823〕

摘　　要：目的:探讨新生大鼠缺氧缺血性脑损伤(HIBD)后大脑皮质不规则趋化因子(CX3CL1)活性的变化及参附注射液对其干预作用。方法:参照Rice法制备新生大鼠HIBD模型,7日龄SD新生大鼠随机分为假手术组(S组)、生理盐水对照组(C组)和参附治疗组(SF组),每一组按照观察时间点的不同,进一步分为6 h、12 h、1天、3天、5天、7天六个亚组,每个亚组8只。C组于造模成功后腹腔注射生理盐水10 ml/kg,1次/天,连用3天,SF组于造模成功后即刻腹腔注射等量参附注射液,S组仅分离右侧颈总动脉后腹腔注射等量生理盐水。免疫组化法和Western-Blot法检测大脑皮质CX3CL1的表达变化。结果:免疫组化法和Western-Blot法显示CX3CL1在S组各时间点表达量均较少,差异无统计学意义(P>0.05);C组和SF组CX3CL1在各时间点的表达水平较S组明显升高,差异有统计学意义(P<0.05),且在3天达到高峰,随后其表达下降;SF组CX3CL1在各个时间的表达水平均低于C组,差异有统计学意义(P<0.05),但高于S组,差异有统计学意义(P<0.05)。结论:HIBD时CX3CL1表达增多,参与脑损伤炎症免疫反应,参附注射液能抑制CX3CL1大量表达从而抑制炎症免疫反应,对HIBD后的新生大鼠可能起到脑保护作用。Objective： To explore the change of CX3CL1 activity in cerebral cortex of neonatal rats with hypoxic-ischemic brain damage （HIBD） and the intervention effect of Shenfu injection. Methods： HIBD model of neonatal rat was established according to Rice method, 7-day SD neonatal rats were randomly divided into sham operation group （S group）, normal saline control group （C group）, and Shenfu injection treatment group （SF group）, the rats in each group were further divided into six subgroups according to observation time points ： 6-hour subgroup, 12-hour subgroup, 1 -day subgroup, 3-day subgroup, 5-day subgroup, and 7-day subgroup, 8 rats in each sub- group. After establishing rat model successfully, the rats in C group were treated with tranabdominal injection of normal saline for consecutive three days, 10 ml/kg, once a day; the rats in SF group were treated with tranabdominal injection of Shenfu injection of the same dose; right common carotid artery of the rats in S group were isolated, then normal saline of the same dose were injected tranabdominally. Immunohisto- chemical method and Western Blot were used to detect the change of CX3CL1 expression in cerebral cortex. Results： Immunohistochemical method and Western Blot showed that the expression levels of CX3CL1 at different time points in S group decreased, there was no statistically significant difference （P〉0. 05） ; the expression levels of CX3CLI at different time points in C group and SF group were statistically signifi- cantly higher than those in S group （ P〈0. 05 ）, which peaked at three days, then the expressi-n levels decreased ; the expression levels of CX3CL1 at different time points in SF group were statistically significantly lower than those in C group （P〈0. 05 ）, which were statistically significantly higher than those in S group （P〈0. 05） . Conclusion： The expression level of CX3 CL1 in cerebral cortex of neonatal rats with HIBD increases, CX3CL1 is involved in inflammatory and immune response of br

关 键 词：新生大鼠 缺氧缺血性脑损伤 不规则趋化因子 参附注射液 

分 类 号：R742[医药卫生—神经病学与精神病学]