Florisil固相萃取法联用气相色谱测定油脂中sn-2位脂肪酸  

Determination of sn- 2 position fatty acid in oils by SPE method with Florisil extraction cartridge coupled with gas chromatography

作　　者：吴琳[1] 刘四磊 魏芳[1] 吕昕[1] 董绪燕[1] 陈洪[1] 

机构地区：[1]中国农业科学院油料作物研究所,农业部油料作物生物学与遗传育种重点实验室,油料脂质化学与营养湖北省重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2015年第2期227-233,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31271879,31171661,21105119);国家支撑计划(2012BAK08B03);国家863计划(2011AA100904);武汉市科技局国际科技合作计划(2013030409020106)

摘　　要：为探寻与气相色谱分析相配套的高效前处理方法,以测定不同物种来源的油脂中sn-2位(β位)脂肪酸组成及含量,考察了Florisil固相萃取柱对经sn-1,3专一性脂肪酶水解后的油脂各产物的分离富集能力,并对萃取条件进行了优化。结果表明,Florisil固相萃取柱能有效地将sn-2单甘脂从油脂水解产物中分离;同时发现Florisil固相萃取可以实现油脂水解产物中甘油三酯、甘油二酯、单甘脂及游离脂肪酸的依次分离。5种不同来源的油脂(植物油、藻油、微生物油脂、鱼油和海豹油脂),水解后经Florisil固相萃取柱萃取,联用气相色谱测定sn-2位脂肪酸组成及含量,结果表明,植物油、藻油和海豹油中sn-2位主要为中长链不饱和脂肪酸(植物油sn-2位中C18∶1,C18∶2和C18∶3约占95%;藻油sn-2位中C16∶1,C18∶1和C18∶2约占50%;海豹油sn-2位中C16∶1,C18∶1和C18∶2约占67%),菌油和鱼油中sn-2位多为长链多不饱和脂肪酸(菌油sn-2位中C20∶4约占37%;鱼油sn-2位中C20∶5,C22∶5和C22∶6约占40%)。Florisil extraction cartridge was tested to purify the enzymes -hydrolyzed oil followed by GC analysis. And the results showed that with Florisil extraction cartridge, sn -2 monoglycerol can be separated from the enzymes - hydrolyzed oil efficiently, and the mixture of triacylglycerol, diaeylglycerol, monoglycerol and free fatty acid can also be efficiently separated in turn at the optimized extraction conditions. On this basis, 5 different types of oils （ plant oil, microbial oil, algal oil, marine animals oil and fish oil） hydrolyzed by sn - 1,3 specificity lipase were purified by Florisil extraction cartridge, then sn -2 monoglycerol were collected before methylating. Qualitative and quantitative analysis of sn - 2 position fatty acid were performed by GC with a FFAP capillary column. The results showed that sn -2 position fatty acids in plant oil, algal oil and seal oil were mainly medium - and long - chain unsaturated fatty acids; sn -2 position fatty acids in microbial oil and fish oil were mainly saturated fatty acids, and in fish oils were mainly long chain polyunsaturated fatty acids.

关 键 词：固相萃取 Florisil固相萃取柱 sn-2单甘脂 

分 类 号：O623.61[理学—有机化学]