HPLC-MS/MS法测定花生中5种常用农药残留量  

Determination of 5 popular pesticide residues in peanut by HPLC- MS / MS

作　　者：庞帅[1,2,3,4] 丁小霞[1,2,3,4] 李培武[1,5,2,3,4] 周海燕[1,2,3,4] 姜俊[1,2,3,4] 邓小伟[1,2,3,4] 都晓慧 

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业部生物毒素检测重点实验室,湖北武汉430062 [3]农业部油料产品质量安全风险评估实验室(武汉),湖北武汉430062 [4]农业部油料及制品质量监督检验测试中心,湖北武汉430062 [5]农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2015年第2期246-249,共4页Chinese Journal of Oil Crop Sciences

基　　金：农业行业科技专项(201303088);国家农产品质量安全风险评估专项(GJFP201506)

摘　　要：为同步检测花生中5种常用农药残留量,采用液-液分配反提取方法,对花生中乙草胺、异丙甲草胺、涕灭威、吡虫啉、辛硫磷等5种农药同步提取、净化与浓缩,高效液相色谱-串联质谱进行测定。结果表明：当加标水平为0.01、0.05、0.10μg/mL时,平均加标回收率为80.9%-108.5%,相对标准偏差为1.5%-7.8%,该方法操作简单,不需要特殊的试剂和仪器,成本低,检出限及定量限低于国家最大残留限量值,检测灵敏度高,各项指标均符合国家农药残留分析质量控制标准,能满足实际样品检测需要。A synchronous detection method by HPLC -MS/MS of 5 pesticide residues in peanut was studied. After being extracted, cleaned - up synchronously with the distribution of liquid - liquid extraction method, aeetoehlor, isopropyl methyl grass amine, aldicarb, imidacloprid and phoxim in peanuts were analyzed by HPLC - MS/ MS. The result showed that at the spiked sample levels of 0.01, 0.05 and 0.10mg/kg, the average recovery rate was 80.9% to 108.5% with the relative standard deviation of 1.5% to 7.8%. This method was simple and eco- nomical without requirement for special reagents and instruments. The detection sensitivity was high with the limits of detection and quantitation both below the national maximum residue limit. All indicators were conformed with the national quality control standards for pesticide residue analysis and this method could meet the requirement of routine analysis of pesticide residues in peanuts.

关 键 词：液相色谱-串联质谱 花生 农药 残留量 

分 类 号：S379.7[农业科学—农产品加工]