白细胞介素-2增强淋巴细胞对乳腺癌细胞的杀伤作用  

Killing effect of interlukin-2 on breast cancer cells by promoting expansion of lymphocytes in vitro

作　　者：熊一全[1] 黄韬[1] 赵向旺[1] 田元[1] 张景辉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院乳腺甲状腺外科,武汉430020

出　　处：《中华实验外科杂志》2015年第5期990-992,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察淋巴细胞以及白细胞介素-2（IL-2）联合淋巴细胞对于激素受体阳性（ER＋）的乳癌细胞株MCF-7的杀伤作用,探讨IL-2增强淋巴细胞的抑癌效应.方法 分别用不同浓度的淋巴细胞以及IL-2加淋巴细胞与乳腺癌细胞株MCF-7共培养,细胞计数试剂盒（CCK-8）法对比分析淋巴细胞对MCF-7细胞的杀伤效应的差异.结果 每孔1 000 U IL-2促进淋巴细胞增殖效应为30.94％;促进淋巴细胞杀伤MCF-7效应为91.24％,增加IL-2的浓度并未增加淋巴细胞杀伤MCF-7能力.结论 IL-2能促进淋巴细胞对MCF-7的杀伤作用.Objective To discuss the inhibitory effect of lymphocytes and interlukin-2 （IL-2）on hormone receptor positive （ER ＋） breast cancer cells MCF-7,and to investigate the mechanism by which IL-2 promotes the killing effects of lymphocytes.Methods Lymphocytes and IL-2 with different concentrations were co-cultured with MCF-7 cells respectively.Cell counting kit-8 （CCK-8） was used to analyze the the viability of cancer cells.Results In MCF-7 cells treated with 1 000 U IL-2,the effect of IL-2 promoting expansion of lymphocytes was 30.94%,and the killing effect of IL-2 on MCF-7 cells by lymphocytes was 91.24%.The killing effect of lymphocytes on MCF-7 cells was not enhanced by increasing the concentration of IL-2.Conclusion IL-2 prompted the tumor inhibitory effect on ER ＋ breast cancer cells MCF-7

关 键 词：白细胞介素-2 乳腺癌 淋巴细胞 

分 类 号：R737.9[医药卫生—肿瘤]