维吾尔族与汉族妇女乳腺癌环氧合酶-2基因在mRNA水平表达差异  

Differential expression of cyclooxygenase-2 mRNA in Uygur and Han women with breast cancer

作　　者：罗琳[1] 李鸿涛[1] 木扎帕·阿不都 马静[1] 宋海涛[1] 马斌林[1] 

机构地区：[1]新疆医科大学附属肿瘤医院乳腺头颈外科,乌鲁木齐830011

出　　处：《中华实验外科杂志》2015年第5期993-995,共3页Chinese Journal of Experimental Surgery

基　　金：新疆维吾尔自治区自然科学基金资助项目（2014211C116）

摘　　要：目的 探讨新疆地区维吾尔族和汉族妇女乳腺癌患者中环氧合酶-2（COX-2）基因在mRNA水平表达差异.方法 收集新疆医科大学附属肿瘤医院详尽的乳腺癌病例资料及新鲜标本120例,应用实时荧光定量聚合酶链反应（FQ-PCR）技术检测癌、癌旁及纤维腺瘤组织中COX-2基因在mRNA表达水平,了解其在维、汉组间及组内表达差异.结果 41例汉族癌、8例癌旁及16例良性组织中COX-2表达水平分别为1.71（0.71 ～6.83）、1.25（0.76 ～ 1.39）、0.42（0.15 ～0.93）;与良性对照组比较,COX-2在癌组织中表达明显升高,差异有统计学意义（P＜0.01）;38例维吾尔族癌、13例癌旁及4例良性组织中COX-2基因表达水平分别为0.41（0.12 ～2.35）、0.95（0.45 ～2.17）、1.33（0.32 ～3.21）,与对照组比较,尚未见COX-2基因在癌组织中表达升高（P＞0.05）;维、汉两组癌组织比较差异有统计学意义（P＜0.01）.结论 乳腺癌中COX-2基因表达较良性组织明显增高,维吾尔族和汉族COX-2表达有种族差异.Objective To investigate the differential expression of cyclooxygenase-2 （COX-2）mRNA in Uygur and Han women with breast cancer in Xinjiang area.Methods Xinjiang Medical University affiliated Tumor Hospital collect detailed information on breast cancer cases and 120 cases of fresh specimens were detected by real-time fluorescence quantitative polymerase chain reaction （FQ-PCR）carcinoma,paraneoplastic and fibroadenoma tissue COX-2 gene expression at the mRNA level,analysis of its expression differences between groups and within groups of Han and Uygur.Results 41 cases of Han cancer,8 cases of paraneoplastic and 16 cases of benign tissue,COX-2 expression levels were 1.71 （0.71 to6.83）,1.25 （0.76 to 1.39）,0.42 （0.15 to 0.93） respectively;compared with the control group,COX 2 expression was significantly higher in cancer tissues,the difference was statistically significant （P 〈0.01）;38 cases of Uygur cancer,13 cases of paraneoplastic and four cases of benign tissue,COX-2 expression levels were 0.41 （0.12 to 2.35）,0.95 （0.45 to 2.17）,1.33 （0.32 to 3.21） respectively,compared with the control group,yet see COX-2 expression in cancer tissues increased （P 〉0.05）;Uygur and Han carcinoma groups difference was statistically significant （P 〈 0.01）.Conclusion COX-2 expression in breast cancer was significantly higher compared with benign tissue,Uygur and Han COX-2 expression was statistically significant,suggesting that COX-2 expression of ethnic differences.

关 键 词：乳腺癌 不同种族 环氧合酶-2 

分 类 号：R737.9[医药卫生—肿瘤]