CXC趋化因子受体4对胆囊癌细胞增殖的影响  

Effects of CXC chemokine receptor 4 on proliferation of primary gallbladder cancer cells

作　　者：侯敬申[1] 王伟雄[1] 黄亮[1] 赵莉[2] 

机构地区：[1]广州医科大学附属第二医院急诊外科,510260 [2]广州医科大学药学院蛇毒研究所

出　　处：《中华实验外科杂志》2015年第5期1039-1041,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金青年科学基金项目（81302357）

摘　　要：目的 观察CXC趋化因子受体4（CXCR4）对人胆囊癌细胞株GBC-SD增殖的影响.方法 分别用实时定量聚合酶链反应（Real-time PCR）、Western blot、流式细胞术等方法检测CXCR4在GBC-SD细胞及人脐静脉内皮细胞株ECV-304中mRNA、蛋白以及细胞表面分子的表达;设计CXCR4靶向短发卡RNA （shRNA）,并构建到pSilencer^Tm2.1-U6质粒中,转染GBC-SD细胞,噻唑蓝比色法（MTT）检测GBC-SD细胞增殖.结果 与ECV-304细胞比较,GBC-SD细胞中CXCR4 mRNA和蛋白呈明显阳性表达,而细胞表面CXCR4分子的表达也明显增高[（70.30±3.50）％与（0.76±0.11）％];与对照组GBC-SD细胞比较,转染了pSilencer^TM 2.1-U6-CXCR4 shRNA质粒的GBC-SD细胞,其CXCR4 mRNA未见明显条带,蛋白表达亦明显降低,MTT结果显示干扰CXCR4 120 h后,GBC-SD细胞吸光度值较未转染组细胞明显降低（3.19±0.16与1.63±0.23）.结论 抑制CXCR4表达可抑制胆囊癌细胞生长.Objective To investigate the effect of CXC chemokine receptor 4 （CXCR4） on the growth of the gallbladder cancer cell line GBC-SD.Methods Using real-time quantitative polymerase chain reaction （Real-time PCR）,Western blotting and flow cytometry,the expression of CXCR4 was detected in GBC-SD cells.After GBC-SD cells were transfected with CXCR4 shRNA,the proliferation was measured by MTT.Results The level of mRNA and protein of CXCR4 in GBC-SD cells,as well as the surface CXCR4 molecule on GBC-SD cells were all highly expressed than that in ECV-304 alls [（70.30 ±3.50）% vs.（0.76 ± 0.11）%].The proliferation of GBC-SD cells was inhibited when CXCR4 was knocked down （3.19 ± 0.16 vs.1.63 ± 0.23）.Conclusion The CXCR4 was highly expressed in GBC-SD cells,and the targeting of stromal cell derived factor-1 （SDF-1）/CXCR4 axis may be a novel strategy to control the progress of gallbladder cancer.

关 键 词：CXC趋化因子受体4 细胞增殖 胆囊癌 

分 类 号：R735.8[医药卫生—肿瘤]