肝外胆管癌组织叉状头/翅膀状螺旋转录因子3阳性调节性T细胞浸润及其临床意义  

Significance of forkhead/winged helix transcription factor P3 ＋ regulatory T cells infiltration in extrahepatic cholangiocarcinoma

作　　者：王文斌[1] 刘三光[1] 刘兵[2] 李月红[3] 

机构地区：[1]河北医科大学第二医院肝胆外科,石家庄050000 [2]河北医科大学第三医院肿瘤科 [3]河北医科大学第二医院病理科,石家庄050000

出　　处：《中华实验外科杂志》2015年第5期1156-1158,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察叉状头/翅膀状螺旋转录因子3（Foxp3）阳性调节性T细胞（Treg）在肝外胆管癌（EHCC）组织中的浸润,探讨环氧合酶-2（COX-2）蛋白表达与Foxp3^＋ Treg细胞浸润的关系.方法 采用免疫组织化学法检测Foxp3^＋ Treg在94例胆管癌组织和COX-2蛋白在77例胆管癌组织中的表达,分析浸润细胞以及COX-2蛋白表达与临床和预后的关系,观察肝外胆管癌组织中COX-2蛋白表达对Foxp3^＋ Treg浸润的影响.结果 94例肝外胆管癌组织中均存在浸润的Foxp3^＋ Treg细胞,中位值为2.35个/高倍视野.Foxp3^＋ Treg细胞的浸润与肝外胆管癌患者的组织学分级、临床分期和淋巴结转移无明显相关（P＞0.05）.但Foxp3^＋ Treg细胞低计数组患者的生存时间明显高于高计数组（P＜0.05）.多因素分析表明Foxp3^＋Treg细胞浸润是肝外胆管癌预后的独立影响因素.77例胆管癌组织中COX-2表达阳性率为81.8％.COX-2蛋白的表达与组织学分级、临床分期和淋巴结转移无明显相关（P＞0.05）.COX-2蛋白表达与Foxp3^＋ Treg细胞浸润之间呈正相关（r=4.89,P ＜0.05）.结论 肝外胆管癌组织中Foxp3^＋ Treg细胞浸润与肝外胆管癌患者的预后负相关,是影响肝外胆管癌预后的独立影响因素.COX-2蛋白表达可能促进Foxp3^＋ Treg细胞的浸润.Objective To explore the infiltration of regulatory T cells （Tregs） and its significance in extrahepatic cholangiocarcinoma （EHCC） and to elucidate the putative effects of cytochrome C oxidase-2 （COX-2） expression on the infiltration of Tregs.Methods The infiltration of forkhead/winged helix transcription factor P3 （Foxp3） ＋ Tregs and the expression of COX-2 were studied by immunohistochemistry in 94 and 77 cases of EHCC respectively.The correlation between the expression of COX-2 and Foxp3^＋Tregs infiltration with clinicopathological features and prognosis was analyzed.The influence of COX-2 expression on infihration of Foxp3^＋ Tregs was evaluated.Results Foxp3^＋ Tregs infiltration was seen in all the 94 cases of EHCC with the median Foxp3^＋ Tregs number being 2.35 per high-power field （HPF）.There was no significant correlation between the number of infiltrated Foxp3^＋ Tregs in cancer tissue and histological grading,TNM stages and lymph node metastasis of EHCC.The patients with less number of infiltrated Foxp3^＋ Tregs showed significantly longer survival than those with greater number of infiltrated Foxp3^＋ Tregs （P 〈 0.05）.Cox analysis resuhs showed that infiltration of Foxp3^＋ Tregs was an independent prognostic factor of EHCC （P 〈 0.05）.The positive expression rate of COX-2 in 77 cases of EHCC was 81.8%.COX-2 expression was not significantly associatied with histological grading,TNM stages and lymph node metastasis of EHCC.COX-2 expression was positively related with stromal Tregs infiltration （r =4.89,P 〈 0.05）.Conclusion The number of infiltrated Foxp3^＋ Tregs in EHCC tissue is negatively related with overall survival of the patients and is an independent prognosis factor of EHCC.COX-2 protein expression may contribute to Foxp3^＋ Tregs infiltration in cancer stroma.

关 键 词：肝外胆管癌 环氧合酶-2 叉状头/翅膀状螺旋转录因子3 调节性T细胞 预后 

分 类 号：R735.8[医药卫生—肿瘤]