SCID小鼠血管瘤模型的建立  

Establishment of the hemangioma model in SCID mice

作　　者：陈淑明[1] 陈少全[1] 张再重[1] 黎成金[1] 夏印[1] 王烈[1] 

机构地区：[1]南京军区福州总医院普通外科,福州350025

出　　处：《中华实验外科杂志》2015年第5期1208-1210,共3页Chinese Journal of Experimental Surgery

基　　金：福建省科技厅社会发展重点项目（2011Y0042）

摘　　要：目的 探讨血管瘤内皮细胞（HemECs）注射法建立血管瘤SCID小鼠模型的有效性.方法 将体外培养的密度为1 ×10^10/L的HemECs悬浮液300μl同基质胶200μl混合注射于SCID小鼠背部皮下,测量注射后第2、15、45天瘤体体积.第45天切取瘤体组织行病理学光镜检查及免疫组织化学染色检测瘤体组织CD31、CD34抗原表达.结果 细胞注射后第2、15、45天瘤体体积为分别为（13.36±2.09）、（49.85±8.24）、（366.57±17.08） mm^3,瘤体组织与原血管瘤生物学特点相似.结论 细胞注射法建立SCID小鼠血管瘤模型方法可靠,操作简单,成活率高.Objective To establish human hemangioma model by injecting subcutaneously hemangioma endotheliar cells （HemECs） into SCID mice.Methods HemECs were resuspended in Matrigel basement membrane matrix,the cells/gel （1.3 × 10^6 cells/200 μl per animal） mixtures were then injected subcutaneously into the back of the SCID mice,and the tumor volume was measured at 2nd,15th,and 45th day in vitro.Mice were sacrificed and samples were taken from survived tumor and stained immunohistochemically by CD31 and CD34 before subjected to histopathological examination at 45th day.Results After cells were injected,the tumor volume at 2nd,15th,and 45th day was （13.36 ±2.09）,（49.85 ±8.24）,and （366.57 ± 17.08） mm^3 respectively.The original tumor histological features were retained in the transplanted tumor.Conclusion It is reliable to establish human hemangioma model by injected subcutaneously HemECs into SCID mice.

关 键 词：血管瘤内皮细胞 模型 

分 类 号：R732.2[医药卫生—肿瘤] R-332[医药卫生—临床医学]