斯钙素2对口腔鳞癌细胞的作用及机制研究  

The role of stanniocalcin 2 in oral squamous cells carcinoma

作　　者：杨舒雯[1,2] 王宇[1] 杨恭 

机构地区：[1]复旦大学附属肿瘤医院头颈外科,复旦大学上海医学院肿瘤学系,上海200032 [2]复旦大学附属肿瘤医院肿瘤研究所,复旦大学上海医学院肿瘤学系,上海200032

出　　处：《中国癌症杂志》2015年第4期269-274,共6页China Oncology

摘　　要：背景与目的:口腔颌面部恶性肿瘤中约80%以上为口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC),虽过去的几十年诊断及治疗技术不断改进,其存活率却没有得到明显改善,5年生存率仍小于50%。该研究旨在探讨斯钙素2(stanniocalcin 2,STC2)对口腔鳞癌细胞KB的增殖、凋亡及侵袭迁移的影响。方法:构建STC2的RNA干扰载体,将其转染KB细胞使STC2基因沉默后,采用CCK8实验检测STC2对KB细胞增殖的影响,通过APC Annexin V/7-AAD染色、流式细胞术检测细胞凋亡情况,分析STC2对细胞凋亡的影响。细胞划痕和细胞小室(Transwell)试验分析比较STC2基因沉默后对口腔鳞癌细胞侵袭和迁移的差异。最后用蛋白[质]印迹法(Western blot)检测凋亡、转移相关蛋白。结果:成功构建KB细胞STC2敲除细胞系,CCK8增殖实验结果显示,STC2沉默后,细胞增殖被明显抑制,其生长速度低于对照组(P<0.001)。在顺铂诱导细胞凋亡过程中,STC2沉默后KB细胞凋亡一定程度被促进。与KB细胞相比,STC2沉默后的细胞迁移和侵袭性明显减弱。Western blot检测发现,沉默STC2后Bcl-2、促细胞迁移和侵袭蛋白Caveolin-1和β-catenin表达下调,bax表达上升。结论:STC2可能参与调控口腔鳞癌细胞KB的增殖凋亡,促进KB细胞的侵袭转移能力,同时一定程度上减弱KB对化疗药物顺铂的敏感性。Background and purpose： About 80% patients with oral and maxillofacial malignant tumor are oral squamous cell carcinoma （OSCC）. OSCC is one of the most common cancers in the world with less than 50% survival rate over 5 years. This experiment aimed to explore the effect of stanniocalcin 2 （STC2） on apoptosis, proliferation, migration and invasion of OSCC cell. Methods： RNA interference （RNAi） vector pLKO. 1-shSTC2 was constructed and transfected into KB cells. Cell proliferation and cell apoptosis were then assessed by CCK8, ＆PC Annexin V/7-AAD and flow cytometry. Differences of migration and invasion between KB scr and KB STC2i were examined by cell scratch and transwell tests. Finally, this study detected the apoptosis-associated proteins and metastasis-associated proteins by Western blot. Results： STC2 down-regulation plasmid was constructed and transfected into KB cells. CCK8 prolifera- tion assay revealed that the STC2 down-regnlation inhibited KB cells proliferation. By treating with cisplatin, this study found that STC2 silence could facilitate cell apoptosis significantly. With the knock down ofSTC2 gene, the expressions of Bcl-2, Caveolin-1 and 13-catenin were decreased but the expression of bax was obviously increased. Conclusion： These data suggest that STC2 may be involved in the apoptosis, proliferation, migration and invasion of OSCC KB cells. Simultaneously, it can significantly weaken the sensitivity of KB cells to chemotherapeutic drug cisplatin.

关 键 词：口腔肿瘤 增殖 侵袭 转移 

分 类 号：R73-37[医药卫生—肿瘤]